Total RNA Extraction from Human Serum Samples

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

TRIzol LS Reagent

Catalog Number:

10296010

The aim of this experiment was to extract total RNA from human serum samples. TRizol LS was mentioned in the literature and hence purchased for the extraction.

Experimental Design and Results Summary

Application

Total RNA Extraction

Starting Material

Serum

Tips

None

Results Summary

Total RNA was successfully extracted. Nanodrop was used to determine the RNA concentration. The total RNA was used for downstream cDNA synthesis and RT-qPCR.

DOI or PMID #

N/A

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Summary

The Good

Very easy to use.

The Bad

None

The Bottom Line

Follow the protocol as mentioned by the manufacturer.

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