Olig-2 Antibody from Sigma Is Very Good

August 07, 2019

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Anti-Olig-2 Antibody

Catalog Number:

AB9610

Clone Number:

Polyclonal

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. I want to stain Oligo2 (Oligodendrocyte transcription factor 2) in mouse corpus callosum cryostat sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse corpus callosum cryostat sections

Primary Incubation

1:100, overnight at 4°C

Blocking Agent

PBST (0.3%) containing 10% goat serum

Secondary Incubation

1:200, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

ZEISS LSM 880 Confocal Laser Scanning Microscope

Results Summary

The signal (red dots) is sharp and specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody works well.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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