Excellent Mounting Media with DAPI for Immunofluorescence from Thermo

National Institute of Immunology, India
Vaccine Immunology Laboratory
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

ProLong™ Gold Antifade Mountant with DAPI

Catalog Number:

P36931

Staining fixed cells or tissue samples with fluorochrome dyes and visualization under microscope within 1-2 days is difficult job. Also visualizing the stained cells or tissue sample for longer time in fluorescence microscope dims/fades the signals of fluorochrome which reduces the quality of picture as well as the confidence on the results. ProLong gold antifade mountant media helps in detecting the fluorochrome signals for longer duration even for months. Only one drop of the reagent is sufficient to mount the fixed cells or tissues.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Fixed cells or any tissue sample

Tips

Also stains nucleus so easy to identify the cells.

Results Summary

Bright fluorochrome signals were observed along with DAPI stained bright blue nucleus.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Economical and easy to use

The Bad

None

The Bottom Line

One of the best mounting media with DAPI to be used for immunofluorescence staining of cells and tissues.

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