Abcam MAT2A Antibody Works Well on Kidney

Pathology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-MAT2A antibody - N-terminal

Catalog Number:

ab189208

To evaluate kidney injury and fibrosis in diabetic nephropathy (negative correlation).

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human kidney

Primary Incubation

1:100 45min

Blocking Agent

Peroxide blocker

Secondary Incubation

Polylink-polyHRP 30min

Tertiary Incubation

N/A

Detection

DAB 10min

Results Summary

To evaluate the association of fibrosis in kidney with MAT2a Ab, we used the Ab first on mice kidney, and when it worked, we tried it on human kidney. We used EDTA for Ag retrieval.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

With 1:100 dilution, large amount of Ab is used with each run.

The Bottom Line

It works well for research purposes.

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