Cell Signaling Technology
S6 Ribosomal Protein
2217S
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Browse Similar Products: S6 Ribosomal Protein Western Blot Antibodies
Since our lab focuses on t cell metabolism, we are definitely interested in looking at t cell activation and phosphorylation of s6. However, we are also interested in looking at the total protein of s6.
Western Blot
Murine T cell
4% BSA in 4C overnight with 1:2500 dilution
5% nonfat milk
5% nonfact milk with 1:5000 rabbit antibody
N/A
ECL
Our results showed that with our drug treatment the total s6 protein drops down in our tumor cells but not in murine t cells.
The antibody works great and is always extremely bright and clean.
The antibody can be too bright so make sure to titrate.
A useful antibody for western if you are interested in activation and s6.