Reliable Antibody for Looking at ACSS1 from Santa Cruz

June 28, 2019

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

ACSS1 Antibody

Catalog Number:

sc-377149

Clone Number:

D-7

Our lab was interested in looking at the acetate level of ACSS1 intake of cells with or without drug treatment on western blot. We used this antibody to probe for ACSS1 and saw that the levels are different with varying treatments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine T cell

Primary Incubation

Overnight in 4C with 1:1000 dilution in 4% BSA

Blocking Agent

5% nonfat milk

Secondary Incubation

1:1000 mouse in room temperature for 1 hr

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Our bands did show up in all samples but there were multiple bands therefore you must look carefully at the respective kDa to distinguish which is the specific band of interest

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use and price was low

The Bad

Multiple bands on western blot

The Bottom Line

Useful if you already know the size of the protein and can distinguish between the bands due to multiple nonspecific bands. It was bright and easy to use in addition to the cost of the antibody being on the lower end.

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