Excellent DAPI for IF from Sigma

Johns Hopkins School of Medicine
Oncology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

DAPI

Catalog Number:

D9542-1MG

Our lab occasionally does microscopy therefore it is always important for us to stain the nucleic acid on our cells in order to visually see where other antibodies are staining.

Experimental Design and Results Summary

Application

IF

Starting Material

Murine T cells

Tips

It is important to make sure the primary and secondary antibodies are washed off.

Results Summary

The DAPI clearly stains the nucleic acids for our cells and there is no nonspecific staining elsewhere on our scope.

DOI or PMID #

N/A

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Summary

The Good

Easy to use and bright

The Bad

It can be too bright if you use too low dilution

The Bottom Line

A useful dye for staining the nucleic acids to distinguish your cell structures.

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