Excellent Antibody for ACSS1 Detection with Western Blot

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

ACSS1 Antibody

Catalog Number:

17138-1-AP

Clone Number:

EPR3915

Our lab was interested in looking at acetate intake on t cells after our glutamine antagonist drug therefore we used this antibody for western blot

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine T cell

Primary Incubation

4% BSA in 4C overnight with 1:2000 dilution

Blocking Agent

5% nonfat milk

Secondary Incubation

Room temperture for 1 hour

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Our results did show an increase in the ACSS1 levels after our drug treatment on our murine cd8 Tcells. There were some unspecific bands but from the kDa we were able to figure out which band was the specific band.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is bright

The Bad

There are some unspecific bands

The Bottom Line

This antibody was great and bright but the unspecific may produce some confusing results. Just have to look at the right kDa.

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