Extraction of Genomic DNA

April 24, 2019

University of Birmingham
ICGS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

PureLink™ Genomic DNA Mini Kit

Catalog Number:

K182001

A kit suitable to extract DNA from different cell types, tissues, etc., with an easy-to-follow protocol.

Experimental Design and Results Summary

Application

To extract genomic DNA from cell lines of interest

Starting Material

Heat block, cell lines of interest, primers for qPCR for the genes of interest

Tips

Ensure to turn on the heat block in advance

Results Summary

One genomic DNA is isolated, qPCR could be performed to quantify the gene of interest in all samples.

DOI or PMID #

N/A

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Summary

The Good

Straight forward protocol with all buffers and columns provided

The Bad

Need to ensure you are using the correct protocol as the manual provides several protocols.

The Bottom Line

Good kit with detailed instructions and a sensitive enzyme that is provided to ensure efficient lysis of the cells. Can be used with frozen samples that have been previously collected/harvested.

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