Sigma
Nitric Oxide Synthase Detection System, Fluorimetric
FCANOS1-1KT
Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform ELISA and western blot assays to determine the role and pro-inflammatory status of macrophages in response to pharmacological inhibition of these kinases. This kit was good to use and contained easy controls to use. I used this to quantify NO from macrophages in culture and response to LPS.
NO detection
N/A
RAW murine macrophages were stimulated and treated with a TAK1 inhibitor at varying concentrations. The cells were then tested for NO production using this kit. The instructions were easy to follow.
Use the controls and experimental set up
This was an easy way to monitor NO production. The kit worked well. The only down fall is there is not the inclusion of a standard curve to understand the amount of NO being made. Rather you can show RFU values.
Easy to use
No standard curve
Works well and good for NO detection.