TaKaRa
NucleoSpin® Gel and PCR Clean-up kit
740609.50
My research focuses on studying posttranslational modification of proteins. I use expressed protein ligation technique to study the effect of phosphorylation and ubiquitination on tumor suppressor protein PTEN and its related NEDD4 HECT E3 ligases. I am also studying the dynamics of ubiquitin-proteasome system using mass spectrometry based proteomics. This clean-up kit is very reliable to purify high quality DNA nucleatides used for cell transfection. I usually use this kit to prepare the single strand DNA templated used as the repair construct for CRISPR knock-in.
DNA purification from PCR or Gel
DNA from PCR or Gel
Load the sample to the column, wash with wash buffer and then elute as instructed.
Use the buffers provided in the kit
The DNA generated using this kit is typically very pure and not that toxic to the cells.
N/A
High quality
A little more expensive.
Definitely use this kit to prepare the single strand DNA template for CRISPR experiments. It will help improve the yield as well.