Great Antibody for a Loading Control (Beta-Actin)

March 18, 2019

Oncology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

β-Actin (13E5) Rabbit mAb

Catalog Number:

4970

My research focus is on understanding the role of the immune system in regulating blood glucose and adipose tissue. For this experiment, HEK293T cells were serum starved and stimulated with insulin. Beta-actin was used as a western blot loading control for the experiment.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T cells

Primary Incubation

4% BSA in TBST overnight at 4°C

Blocking Agent

5% milk in TBST

Secondary Incubation

5% milk in TBST for 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

HEK293T cells were serum starved and stimulated with insulin. Beta-actin was used as a protein loading control for the experiment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great signal detection

The Bad

Need to dilute antibody because very bright signal

The Bottom Line

This is a great antibody for beta-actin detection for a loading control for a western blot. However, you need to dilute the antibody because the signal strength is strong.

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