beta-Actin (13E5) Rabbit mAb 4970L from Cell Signaling Technology

Product Specs
Itembeta-Actin (13E5) Rabbit mAb
CompanyCell Signaling Technology
Price $869.00 Inquire
Catalog Number4970L
Quantity300 µl
ApplicationsW, W-S, IHC-P, IF-F, IF-IC, FC-FP
ReactivityH, M, R, Mk, B, Pg
Targetbeta-Actin
HostRabbit
NCBI Gene AliasesBRWS1, PS1TP5BP1
ClonalityMonoclonal
NCBI Full Gene Nameactin beta
Research AreaCytoskeletal Signaling
Clone1300000
IsotypeIgG
Molecular Weight45

Questions and Answers

Can this antibody be used for Western blotting in various cell types?

Yes, β-Actin (13E5) Rabbit mAb has been shown to be effective for Western blotting applications, as evidenced by its use as a loading control in multiple studies, including those involving myelogenous leukemia cell lines and various other cell types (, ).

Does this antibody cross-react with other actin isoforms?

No, β-Actin (13E5) Rabbit mAb specifically detects endogenous levels of total β-actin protein and does not cross-react with cytoplasmic γ-actin or any other actin isoforms, making it a reliable choice for studies requiring specific detection of β-actin.

What species can this antibody be used on?

This antibody is reactive with multiple species, including human, mouse, rat, non-human primates, bovine, and porcine, making it versatile for various experimental models.

Is there any evidence of this antibody being used in immunohistochemistry?

Yes, β-Actin (13E5) Rabbit mAb has been utilized in immunohistochemistry, as indicated by its application in studies assessing protein expression in fixed, paraffin-embedded tissues ().

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Peptidoglycan from Bifidobacterium adolescentis enhances IL-10 production in regulatory B cells to alleviate gut inflammationGut MicrobesDecember 31, 2026Sohyeon Lee, Yoonho Lee, Ho-Su Lee, Jiyoung Yu, Kyunggon Kim, Tae-Young Kim, Su-Hyun Lee, Yuan Qiao, Seungil Kim, Mi-Na Kweonanti-ERK (#9102), anti-phospho-ERK (#9101), anti-p38 (#9212), anti-phospho-p38 (#9211), and anti-β-actin (#4970) (all from Cell Signaling Technology, Danvers, MA). After washing, membranes were incubated with HRP-conjugated

(2) Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitisGut MicrobesDecember 31, 2026Vinita Kushwaha, Beng San Yeoh, Piu Saha, Benoit Chassaing, Bina Joe, Andrew T Gewirtz, Matam Vijay-KumarSigma (St. Louis, MO). Antibodies to MFN1 (Catalog #14739), OPA1 (Catalog #80471), β-actin (Catalog #4970T), pAMPK (Catalog #2531), AMPK (Catalog #2532), and Hif1α (Catalog #79233T) were procured from Cell Signaling Technology

(3) Prophylactic quercetin administration attenuates pulmonary fibrosis via ferroptosis-resistant priming of alveolar epithelial cellsRedox ReportDecember 31, 2026Yang Qiao, Xie Cheng, Zixin Luo, Weilu Huang, Zheng Zhang, Kangkang Ren, Xinping Xu, Huan He, Ming He, Lu Yin(Cell Signaling Technology, 12721), Phospho-NRF2-S40 (ABclonal, AP1133), β-actin (Cell Signaling Technology, 4970), GAPDH (Proteintech, 60004-1-Ig), and H3 (Proteintech, 81984-2-RR) were used at a dilution of 1:1000

(4) Sialic acid cis-ligand dynamics modulate Siglec-7 and -9 function and affect Siglec-7/9 co-blockade to potentiate natural killer cell anti-tumor activityOncoimmunologyDecember 31, 2026Jesper van Eck van der Sluijs, Anne H. C. Valk, Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike Looman, Willemijn Hobo, Anna E. Marneth, Harry Dolstra, Lune van den Boogaard, Christian Büll, et al.anti-Sialyl-Tn (Abcam, Ab115957, RRID: AB_10901541) and 1:1000 rabbit-anti-β-actin (Cell Signaling, 4970S, RRID:AB_2223172) in 1:4 blocking solution in PBS-T overnight at 4 °C, respectively. After being washed

(5) Responses of intestinal organoids to infection by Mycobacterium avium resemble symptoms observed in Crohn’s diseaseGut MicrobesDecember 31, 2026Wanbin Hu, Adriana Martinez Silgado, Ninouk Akkerman, Ronald W.A.L. Limpens, Roman I. Koning, Hans Clevers, Herman P. Spainkand incubated overnight at 4 °C with primary antibodies MMP7 (3801, Cell Signaling D4H5) and β-actin (4970, Cell Signaling 13E5) (loading control). After washing, membranes were incubated with HRP-conjugated

(6) Hemoglobin inhibits fibroblast-to-cardiomyocyte reprogramming via TLR2/TLR4-dependent chromatin compactionMolecular Therapy Nucleic AcidsJune 16, 2026Iqra Anwar, Xinghua Wang, Richard E. Pratt, Victor J. Dzau, Conrad P. Hodgkinsonantibodies used were Gapdh (Cell Signaling, catalog no. 5174), β-actin (Cell Signaling, catalog no. 4970S), phospho-NF-κB (Cell Signaling, catalog no. 3033S), and phospho-p38 MAPK (Cell Signaling, catalog no.

(7) PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growthTumour Virus ResearchJune 1, 2026Shuxiang Wu, Xiaozhen Chen, Yilin Huang, Fangrong Zhang, Xinjian Lin, Xu Lin(Cell Signaling Technology, #14793S), BIP (Cell Signaling Technology, #3177), β–actin (Cell Signaling Technology, #4970S), PRMT5 (Cell Signaling Technology, #2252S), monomethylarginine (IMMUNECHEM, #ICP0801), symmetric dimethyl

(8) Targeting ROR1 with humanized antibody drug conjugates and cytokine fusion proteins for cancer therapyiScienceMay 15, 2026Beixue Li, Rui Jin, Ziping Qi, Fengming Zou, Shuang Qi, Aoli Wang, Li Wang, Qingsong Liu, Chen Hu, Wenchao Wang, et al.,Cell Signaling Technology,Cat#4102; RRID: AB_2180136 Beta-Actin (13E5) Rabbit Monoclonal Antibody,Cell Signaling Technology,Cat#4970; RRID: AB_2223172 Anti-Rac1 Antibody,Abcam,Cat#ab97732; RRID: AB_10679979 Anti-LAMP-2 Antibody,Abcam,Cat#ab125068;

(9) Small extracellular vesicles derived from hair follicle neural crest stem cells enhance perineurial cell proliferation and migration via the TGF-β/SMAD/HAS2 pathwayNeural Regeneration ResearchMay 1, 2026Yiming Huo, Bing Xiao, Haojie Yu, Yang Xu, Jiachen Zheng, Chao Huang, Ling Wang, Haiyan Lin, Jiajun Xu, Pengfei Yang, et al.Cat# DF13702, Affinity, Cincinnati, OH, USA), rabbit monoclonal anti-β-actin antibody (1:1000, Cat# 4970, Cell Signaling Technology), and mouse monoclonal anti-β-tubulin antibody (1:5000, Cat# M20005, Abmart,

(10) A covalent allosteric molecular glue suppresses NRF2-dependent cancer growthCancer DiscoveryMay 1, 2026Nilotpal Roy, Tine Wyseure, I-Chung Lo, Justine Lu, Christie L. Eissler, Steffen M. Bernard, Ilah Bok, Aaron N. Snead, Albert Parker, U-Ging Lo, et al.dilution; Cell Signaling Technology, # 20733);β-actin (1:50 dilution; Cell Signaling Technology, # 4970); andKEAP1 (1:50 dilution; Cell Signaling Technology, # 8047). Cell Lines and Culture Cell Line Source The

(11) Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polypsActa Otorhinolaryngol ItalApril 30, 2026Yu Zhong, Huijuan Yan, Yuren Zhou, Nanlan Huang, Jingkun Liphosphorylated NF-κB (p-NF-κB) (CST, 3033S), phosphorylated MAPK (p-MAPK) (CST, 4370S), and β-actin (CST, 4970S) as an internal control. After incubation with HRP-conjugated secondary antibodies (CST, 7074S), protein

(12) THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleeLifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colón, Carine Bossard, et al.Cell Signaling, #3300), P21 (1:1000; Cell Signaling, #2947 S), and β-actin (1:1000; Cell Signaling, #4970 S). After washing with 5% TBST, the membranes were incubated with horseradish peroxidase-conjugated

(13) Gastroprotective [6]-Gingerol Aspirinate as a Novel Aspirin-Derived Chemopreventive Agent Attenuating ColitisJ Agric Food ChemApril 29, 2026Pei-Sheng Lee, Shuwei Zhang, Yingdong Zhu, Vadin Ha, Shengmin SangGAS was synthesized in our laboratory. The mouse anti-β-actin monoclonal antibody (catalog number: 4970S) was purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-COX-2 monoclonal antibody

(14) IRS4 is a PI3K-activating cancer dependency up-regulated through DNA rearrangements or epigenetic mechanisms in multiple solid tumorsSci AdvApril 29, 2026Khadija Banu, Mohammad Aslam Khan, Sihan Li, Morgan Reynolds-Gagliano, Xiaofei Wang, Robert J. Mobley, Kelly R. Barnett, Matthew M. Wielgosz, Matthew Bauler, Christopher Wincek, et al.Serine 473 (Cell Signaling, no. 4060), Akt (Cell Signaling, no. 4691), β-Actin (Cell Signaling, no. 4970), PIK3R1 (p85) (4292S), SMARCB1 (Cell Signaling, no. 91735), or FLAG tag (Abcam, EPR20018-251), followed

(15) LGALS3BP Links Centrosomes and Mitochondria to Maintain Energetic Fitness and Restrain Compensatory Lipid Catabolic Reprogramming in Hepatocellular CarcinomaCancer Genomics ProteomicsApril 29, 2026Jun Eul Hwang, Hyun Jeong Shim, Hyun Jin Bang, Hyeon-Jong Kim, Sung Yun Jung, Ik-Joo Chung, Sang-Hee Cho, Dae-Hwan Kimprimary antibodies: anti-phospho-AMPKα (Thr172), anti-total AMPKα, and anti-β-actin (#2535, #5832, and #4970; all from Cell Signaling Technology, Danvers, MA, USA). Detection was carried out using HRP-conjugated

(16) Pan-cancer ex vivo target evaluation of phosphodiesterase 3 A (PDE3A)J Mol Med (Berl)April 28, 2026Kiesha Rice, Noora Lehtinen, Eetu Välimäki, Janne Suhonen, Pekka Taimen, Kimmo Kettunen, Sami Ventelä, Juha Kononen, Harri Sihto, Juha K. Rantalameasurement, the arrays were stained with a Sypro Ruby Blot solution (Invitrogen) and an antibody for actin (#4970, Cell Signaling Technology). The slides were scanned with a Tecan LS Reloaded (Tecan) microarray scanner

(17) Targeting STAT5A via CRISPR/Cas9 restores TKI sensitivity in resistant chronic myeloid leukemia cellsMed OncolApril 25, 2026Besne Çelik, Yağmur Kiraz, Yaren Şahin, Burçin Tezcanlı Kaymaz(Upstate Cell Signaling Solutions, Cat. No. 06–968) and β-actin (Cell Signaling Technology, Cat. No. 4970), followed by incubation with HRP-conjugated secondary antibodies. Signal detection was performed using

(18) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencyNat CommunApril 24, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguram(Cell Signaling Technology; 14697, 1:1000 dilution) and rabbit anti-actin (Cell Signaling Technology; 4970, 1:1000 dilution). The membrane was washed three times with 1×TBST (150 mM NaCl, 0.5% Tween-20, and

(19) Age-Dependent Alterations of Chromosomal Passenger Complex Members During Implantation and Decidualization in the Mouse UterusAging CellApril 23, 2026Ezgi Golal, Cemre Nur Balci, Nuray AcarpIncenp, Aurora Kinase B, and Borealin. The membranes were also treated with Beta‐actin antibody (#4970, Cell Signaling Technology, Danvers, MA, USA) as an internal control. The bands were measured using

(20) Overexpression generates aberrant distribution of endocytic regulators - the case of the Rab11/LAMP1 compartmentPLoS OneApril 22, 2026Sarah Hornfeck, Evgeniya Trofimenko, Christian Widmann11814460001, RRID:AB_390913) was used at 1:1000 for WB. The anti-β-Actin antibody (Cell Signaling Technology, 4970, RRID:AB_2223172) was used at 1:1000 for WB. The FITC conjugated anti-mouse antibody (Jackson Immunoresearch,

Write a Review

(1)

9/16/2020Useful, Well Working Loading Control for Western Blotting Saskia HainbuchWorks great and always gives a clean signal.
(1)

3/18/2019Great Antibody for a Loading Control (Beta-Actin) Matthew ArwoodThis is a great antibody for beta-actin detection for a loading control for a western blot. However, you need to dilute the antibody because the signal strength is strong.
(1)

2/20/2019Western Blot of HeLa Whole-Cell Lysates Using Actin-Specific AntibodyDr. Raghavendra VadlaOur aim was to examine the protein levels of our work under untreated and bleomycin (which induces double-stranded breaks) treated HeLa cells. We extracted whole-cell lysates from cells using the ... Read Review
(1)

2/13/2019Great Antibody for Actin Hong SunWe commonly use beta-actin as a loading control for immunoblot across all treatment conditions. The antibody is easy to use and works well.
(1)

11/15/2018Actin for Normalizing Western Blot Meng SunOur lab is interested in comparing the mTOR, epigenetic, and metabolism signals of T cells in multiple media conditions with varying concentrations of glutamine. We use different concentrations to ... Read Review

beta-Actin (13E5) Rabbit mAb

beta-Actin (13E5) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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beta-Actin (13E5) Rabbit mAb from Cell Signaling Technology

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