Good Reagent for Detecting Gene Expression in Human Lung Samples

Princeton
Molecular Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Takara

Product Name:

Human lung total RNA

Catalog Number:

636524

This product comes from three deceased individuals (low number) and is useful for assessing gene expression by qPCR. Here we were comparing the expression of several endothelial specific genes across disease states.

Experimental Design and Results Summary

Application

Reverse transcription and qPCR

Starting Material

N/A

Protocol Overview

This RNA product was reverse transcribed with the superscript IV system, and then conventional q-RT-PCR was performed on CD31 along with other endothelial-specific genes. The peaks and variability were of high-quality as shown by the low cycle number for CD31.

Tips

Use with clontech 636591 and 636531 for assessment of lung, liver, and bone

Results Summary

Results demonstrated that this product had intact mRNA that was in good shape as CD31 was readily detected by qPCR as well as lower abundance products.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy assessment of lung gene expression

The Bad

Only 3 subjects is too low to assume pooled expression

The Bottom Line

This along with the clontech products is an ideal way to assess gene expression across healthy human organs.

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