Easy and Rapid Golgi Stain Kit

Jali Medical Inc.
Physiology
Research Scientist

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Company:

FD Neurotechnologies, Inc.

Product Name:

FD Rapid GolgiStain™ Kit

Catalog Number:

PK401

Golgi impregnation made easy using this kit where all solutions are provided. The kit has been used to stain brain sections from P15–80 aged mice brains for good quality stain of neuronal dendrites and spines.

Experimental Design and Results Summary

Application

Imaging Neurons and Dendritic Spines

Starting Material

Mouse brain sections

Protocol Overview

Follow as directed in the manual provided. Mouse brains can be stored for longer periods of time in Solution C and refrigeration but Solution C should be changed often as recommended by the manual.

Tips

Use refrigerated Solution C in sectioning using a vibratome.

Results Summary

The kits allow flexibility on tissue processing as you can section using a cryostat, vibratome, or microtome. A pink brain will often produce lower quality staining because of vessels being stained.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Time saving

The Bad

Some solutions are used more and are depleted more rapidly

The Bottom Line

The kit works well with brain sections anywhere between 100–300 micron thickness for all mouse ages.

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