H2DCFDA Detects Intracellular ROS Levels in 15d-PGJ2 Treated Cardiomyocytes

January 17, 2019

Medical University of Graz
Department of Cardiology
Postdoctoral Researcher

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Company:

Tocris Bioscience

Product Name:

H2DCFDA

Catalog Number:

5935/100

HL-1 cardiomyocytes were stimulated with 15d-PGJ2 up to 60 min to follow intracellular ROS levels using H2DCFDA.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Cardiomyocytes

Protocol Overview

HL-1 cardiomyocytes were incubated with 10 µM H2DCFDA for 30 min.

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Results Summary

H2DCFDA detects intracellular ROS levels in 15d-PGJ2 treated cardiomyocytes

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Summary

The Good

Works as expected

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The Bottom Line

H2DCFDA detects intracellular ROS levels in 15d-PGJ2 treated cardiomyocytes.

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