PE-Isotype Control Antibody

Center for Regenerative Medicine
Boston University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Rat IgG2A PE-conjugated Antibody

Catalog Number:

IC006P

The aim of this experiment was to sort out a specific selection of cells using PE-conjugated antibody. The Rat IgG2A PE-conjugated Antibody was recommended by the R&D systems.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cells from total rat lung digest

Primary Incubation

10 ul/test- test=100 ul of sample

Blocking Agent

Not applicable

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Laser

Results Summary

The PE flurophore was very dim.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

None

The Bad

Very dim, doesn't stain cells well for control.

The Bottom Line

The PE-dye is very dim. Needs several optimization trials.

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