Invitrogen
CD4 Monoclonal Antibody (RM4-5), FITC
11-0042-85
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We used this product to study immune cells during an antigen exposure in mouse model. In an immunology laboratory use of FACS antibody is very common to distinguish different cell populations present in the splenocytes/lymphocytes and increase and decrease in the expression of different surface markers on it. In our study we used CD4-FITC antibody to study T-helper cells. This antibody fits best in our antibody cocktail for multi-color flow cytometry. The antibody is efficient and works well.
Flow Cytometry
Mouse splenocytes/lymphocytes
1:800 dilution, 4°C, 30 minutes for 50 µl test sample (1-2 X 106 cells)
Fc-block
None
Flow cytometry
Separate cloud of FITC positive cells were observed indicating CD4+ T cells.
N/A
Same antibody can be use to detect CD4+ T-cells present in mouse blood.
It works at lower concentration efficiently, which allows us to use it for more tests than is indicated in the antibody flyer.
Excellent antibody to use for multi-color flow cytometry.