Good Antibody for p62 Detection

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling Technology

Product Name:

SQSTM1/p62 Rabbit Monoclonal Antibody

Catalog Number:

39749

Cell lysates are collected from different stimuli and western blot analysis is carried out to determine changes in p62 expression. The antibody is sensitive and produces sharp bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Macrophages Cell Line

Primary Incubation

1:1000 overnight at 4°C

Blocking Agent

1% Milk

Secondary Incubation

IRDye 680RD Goat anti-Mouse Secondary Antibody (0.1 mg)

Tertiary Incubation

NIl

Detection

Fluorescence

Results Summary

The bands produced are sensitive and sharp.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and produces good results

The Bad

Can only be used for IP and WB

The Bottom Line

A good quality antibody for those who are carrying out studies regarding p62.

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