Anti-UBAP2 for Western Blot

January 04, 2019

BlueskyReddit
LMP
University of Toronto
Graduate Student

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Company:

Abcam

Product Name:

Anti-UBAP2

Catalog Number:

ab197083

Browse Similar Products: UBAP2 Immunoprecipitation Antibodies

UBAP2 is a ubiquitin-related protein that is reported to play a role in the ubiquitination pathway. Here we assess the expression of UBAP2 in the PC3 prostate cancer cell line. The antibody gives a strong band for the protein of interest.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

PC whole-cell lysate

Primary Incubation

5% skimmed milk - 15 min room temperature

Blocking Agent

1:1000, Overnight at 4°C

Secondary Incubation

1:10000, anti-rabbit HRP conjugated antibody, 30 min at room temperature

Tertiary Incubation

N/A

Detection

ECL - film

Results Summary

Antibody worked alright and was able to detect the protein of interest when comparing knockdown and control cell lysates. Size ~ 120 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong band observed for protein of interest!

The Bad

There were a few non-specific bands below the target band that were present but did not interfere with the protein of interest

The Bottom Line

Works well at detecting UBAP2 with minimal background bands.

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