Thermo Fisher Scientific
Trypan Blue Solution (0.4%)
15250061
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We manually count viable and non-viable cells using a hemacytometer. Trypan blue allows us to determine the cell concentration by staining live cell membranes but accounts dead cells by staining them blue. The product is easy to use and works well.
Cell culture counting
Single Cell Suspensions of any cell types
We dilute the trypan blue (0.4%) 1:10 in PBS for a final 0.04% solution since the concentrated version is too strong but also wasteful.
Diluting trypan solution in PBS saves money.
We are able to distinguish viable and non-viable cells using trypan blue and hemacytometer.
N/A
Make sure you mix well.
Easy to use and can easily tell live versus dead cells. Cheap.
Can stain clothes if not careful.
Wonderful viability solution to help manual counting. Easy to use and reliable over time.