Great Antibody to Detect GATA-6 Expression

Oncology
Johns Hopkins School of Medicine
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

GATA-6 (D61E4) XP® Rabbit mAb

Catalog Number:

5851

We study tissue-resident macrophages. To define a specific subset of peritoneal macrophages, we analyze expression of the transcription factor GATA-6 as a marker for this population by immunoblotting. The antibody is reliable.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine Macrophages

Primary Incubation

1:2500 in 4% BSA/TBST

Blocking Agent

5% dry milk/TBST

Secondary Incubation

1:5000 IgG Rabbit HRP in 5% dry milk/tbst

Tertiary Incubation

None

Detection

Invitrogen ECL Pico

Results Summary

We are easily able to determine GATA-6 by western blot analysis especially since we observe expression in the expected condition and not the other.

DOI or PMID #

28877476

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable antibody used at a high dilution for cost saving

The Bad

None really

The Bottom Line

Great antibody to detect GATA-6 expression by western blotting.

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