Great Antibody for IF

December 18, 2018

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

Anti-HA antibody produced in rabbit

Catalog Number:

H6908

Browse Similar Products: HA Tag Immunofluorescence Antibodies

We wanted to detect HA-tagged Vimentin using immunofluorescence. This antibody was highly specific and how low background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

MCF-7 human breast cancer cells

Primary Incubation

1:200 dilution of the antibody

Blocking Agent

5% goat serum in PBS

Secondary Incubation

Goat Anti-rabbit AF488

Tertiary Incubation

None

Detection

Confocal microscope

Results Summary

Nice filament structure was able to be detected with the antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific. Relatively low background.

The Bad

None

The Bottom Line

Great antibody to use in both IF and WB.

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