Rapid and Easy Cell Proliferation Reagent

Princeton
Molecular Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Alstem Bio

Product Name:

EZQuant

Catalog Number:

CA01

The easiest cell proliferation/cytotoxicity reagent I have used. Rapid, sterile, and low-variability measurement of NADH levels in living cells that allows for repeat measurements and hence paired statistical testing.

Experimental Design and Results Summary

Application

Cell proliferation

Starting Material

Human cultured cells

Protocol Overview

The reagent is added into 96-well plates and allowed to incubate for 1–3 hours. Absorbance is measured on a plate reader, then the media can be replaced and the cells will continue growing, ready for a read the following day.

Tips

Reagent can be diluted 1:5 and still works well.

Results Summary

Measures NADH as a proxy for viability.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

One-step process

The Bad

Need to order repeatedly (small 5 mL size)

The Bottom Line

Best cell proliferation reagent for cell culture. One-step process with great sensitivity and low variability.

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