Mouse IL-18Ralpha A647, Flow Cytometry

December 05, 2018

Innate Immunity Department
German Rheumatism Research Center Berlin
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 647 anti-mouse CD218a (IL-18Rα) Antibody

Catalog Number:

132903

I am interested in innate lymphoid cells of different organs and thus need antibodies to identify populations. IL-18Ralpha is expressed on mouse NK cells isolated from lung tissue. The antibody was good for identifying NK cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung lymphocytes

Primary Incubation

IL-18Ralpha A647, clone BG/IL18RA; 15 min at 37°C in PBS/BSA

Blocking Agent

Anti FcgR, clone 2.4G

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Symphony

Results Summary

C57BL/6 mouse NK cells are stained positive with anti IL-18Ralpha A647

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice resolution for cytokine receptor

The Bad

Nothing bad to say

The Bottom Line

Good antibody, will use it in addition to other markers to identify NK cells

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