T100 Thermal Cycler for PCR

Johns Hopkins School of Medicine
Oncology
Lab Technician

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

T100™ Thermal Cycler

Catalog Number:

186-1096

This thermal cycler is effective and reliable for every time we want to run PCR. The small size makes it easy for us to put on a bench top. The touchscreen also makes it easy to change settings and select for customizable protocols.

Experimental Design and Results Summary

Application

PCR

Protocol Overview

We combined the DNA obtained from mouse tail and did a PCR reaction in 8-tube PCR strips. Since we genotype our own mice, we require multiple strips for quick PCR.

Tips

Leaving the machine overnight is also doable

Features Summary

Make sure to turn off the PCR and let it cool a bit otherwise it may overheat

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliable and efficient and also easy to use

The Bad

Overheating might be an issue

The Bottom Line

User-friendly PCR machine and quick

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