Miltenyi Biotec
CD4+ T Cell Isolation Kit, mouse
130-104-454
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We are interested in the CD4-specific population after we harvest splenocytes from mice. We use this kit all the time when we want a good population. The populations are always very clean.
Isolation of CD4+ population
Spleen and lymph node from mice
We let the cells sit with the antibodies with MACS buffer on ice, according to the protocol of the manufacturer.
Keep everything on ice
Our population is always very clean, with yield of about 90% CD4+ cells.
N/A
Clean isolation and clean population
Costly
This isolation kit is very useful for a clean CD4+ population.