Miltenyi Biotec
CD8a+ T Cell Isolation Kit, mouse
130-104-075
Info:Supplier Page View Product Specs View Product
Our lab is interested in isolating CD8 T cells after harvesting spleens from mice. After isolation, we wanted to look at the CD8 T cell gene expression under varying conditions of glutamine concentration. The isolation was reliable at times but not clean other times.
CD8 T Cell Isolation
Splenocytes
After harvesting the spleens, we grind the spleen against a nylon mesh. Then we follow the manufacturer's protocol to isolate CD8s.
Keep everything on ice
The isolation was reliable at times but also not clean at other times. Our yield was about 90% CD8s. We left the antibodies for longer than the protocol recommended because we think maybe the binding isn't as efficient as before. It worked better after we left it longer.
N/A
Very clean isolation for the most part, for both negative and positive
Price is always high per isolation run
Definitely clean isolation and very usable for mice samples