STIM1 Antibody

Pediatrics
Drexel University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

STIM1 Antibody

Catalog Number:

4916

Stromal interaction molecules (STIMs) function as Ca2+ sensors to detect changes in Ca2+ content in intracellular Ca2+ stores. STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors. This antibody works well to detect STIM1 by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse heart

Primary Incubation

STIM-1 (1:1000 dilution), Overnight at 4°C

Blocking Agent

Odyssey blocking buffer (1:1)

Secondary Incubation

Goat anti-Rabbit IgG, LICOR (1:5000 dilution) at RT for 1 hr

Tertiary Incubation

N/A

Detection

Scanned on Odyssey® CLx (auto scan 700 & s800)

Results Summary

STIM-1 antibody from Cell Signaling Technology is good to measure STIM-1 expression by immunoblot.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good

The Bad

N/A

The Bottom Line

STIM-1 antibody at 1:1000 dilution works very well. I recommend this antibody for western blot.

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