STIM1 Antibody 4916S from Cell Signaling Technology

Product Specs
ItemSTIM1 Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number4916S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk
TargetSTIM1
HostRabbit
NCBI Gene AliasesD11S4896E, GOK, IMD10, STRMK, TAM, TAM1
NCBI Full Gene Namestromal interaction molecule 1
ClonalityPolyclonal
Research AreaCa, cAMP and Lipid Signaling
Molecular Weight85

Questions and Answers

Can this STIM1 antibody be used to study store-operated calcium entry in fibroblasts?

Yes, the STIM1 antibody has been shown to be effective in studies investigating store-operated calcium entry (SOCE) in primary murine lung fibroblasts. Research indicates that STIM1 is crucial for SOCE, and its deficiency leads to decreased cell proliferation and migration in these cells ().

Is this antibody suitable for use in Western blotting applications?

The STIM1 antibody has been utilized in Western blotting, as evidenced by its application in various studies, including those examining the phosphorylation status of STIM1 and its role in calcium signaling pathways (, ).

What species can this antibody detect, and is it effective in human studies?

The STIM1 antibody is reactive with multiple species, including humans, mice, rats, and non-human primates. It has been effectively used in studies involving human cells, particularly in the context of breast cancer research where STIM1 expression is regulated by microRNAs ().

Are there any known interactions of STIM1 with other proteins that this antibody can help elucidate?

Yes, STIM1 has been shown to interact with various proteins involved in calcium signaling, such as Orai channels. Additionally, studies have indicated that STIM1's localization and function can be influenced by other proteins, such as RASSF4, which regulates its activity at the endoplasmic reticulum-plasma membrane junctions ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A Quartet of Native Orai Channel Isoforms Orchestrates Graded NFAT Activation and TranscriptionbioRxivJuly 13, 2026Ahmed Emam Abdelnaby, Yin-Hu Wang, J. Cory Benson, Isagani Perez, Mark Shen, Maxwell McDermott, Miki Jishage, Amal T. Elhaw, Silvia Cruz-Rangel, Raphael Courjaret, et al.T cells (see main manuscript methods) were incubated with a STIM1 primary antibody overnight at 4°C (4916S, Cell signaling,) and for 2 hours at room temperature with an Ax555 coupled secondary antibody (Molecular

(2) Ca 2+ influx through ER-plasma membrane contacts is required for brown fat thermogenesis and metabolic healthbioRxivMarch 26, 2026J Zhou, C Dogan, L L Artico, K Rodrigues, S Hakam, T Kim, V Xu, K Lapenta, M Kang, D M Jorgens, et al.using the enhanced chemiluminescence system (Bio-Rad). Antibodies used were STIM1 (Cell Signaling, 4916), STIM2 (Cell Signaling, 4917), α-Tubulin (Proteintech, 66031-1-Ig), β-Tubulin (Cell Signaling, 2146S),

(3) STIM1-Mitofusin2 interactions tether mitochondria and melanosome contacts that promote melanosome maturationNature CommunicationsMarch 6, 2026Isshin Shiiba, Yuto Ishikawa, Hijiri Oshio, Naoki Ito, Fuya Yamaguchi, Shun Nagashima, Hideya Ando, Keitaro Umezawa, Yuri Miura, Yuhei Araiso, et al.1:1000), streptavidin–HRP (Vector Laboratories, SA5004; 1:1000), STIM1 (Cell Signaling Technology, 4916S; 1:1000 for immunoblotting, 1:500 for immunofluorescence), GAPDH (Santa Cruz Biotechnology, sc-32233;

(4) TSC-associated microglial hyperactivity: enhanced calcium signaling, metabolism, and phagocytosisActa NeuropatholFebruary 13, 2026Rozemarijn S. Kalf, Mark J. Luinenburg, Giulia Dematteis, Mirte Scheper, Jasper J. Anink, Giulia Cavallo, Andrea Mattarei, Wim Van Hecke, Angelika Mühlebner, Laura Tapella, et al.Anti-IP3R (Immunological Science AB-82747), Anti-SERCA2 (abcam ab255960), Anti-STIM 1 (Cell signaling, 4916), Anti-ORAI1 (Invitrogen PA-74181); Ponceau S Solution (Sigma P7170), and Anti-β-actin (Sigma-Aldrich

(5) Identification of Novel Kv1.3 Channel-Interacting Proteins Using Proximity Labelling in T-CellsCellular Physiology and BiochemistryOctober 31, 2025Department of Neurology, School of Medicine, Yale University, New Haven (CT), USA, Dilpreet Kour, Christine A. Bowen, Center for Neurodegenerative Diseases, Emory University, Atlanta (GA), USA, Department of Biochemistry, Emory University, Atlanta (GA), USA, Upasna Srivastava, Hai M. Nguyen, Rashmi Kumari, Prateek Kumar, Amanda D. Brandelli, et al.,Cell Signaling Technology,80076S Anti-V5 tag antibody,Abcam,ab206566 Anti-STIM antibody,Cell Signaling Technology,4916S Anti-CD3 epsilon,Cell Signaling Technology,85061S Anti-NF-ATc1 antibody,BD Biosciences,556602 Anti-calnexin

(6) Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migrationCell ReportsApril 22, 2025Holly Merta, Kaitlynn Gov, Tadamoto Isogai, Blessy Paul, Achinta Sannigrahi, Arun Radhakrishnan, Gaudenz Danuser, W. Mike HenneAB_2880526 Rabbit anti-GAPDH,Abcam ab9485,RRID:AB_307275 Rabbit anti-STIM1,Cell Signaling Technologies 4916,RRID:AB_2271287 Goat anti-Rabbit HRP,Abcam ab6721,RRID:AB_955447 Rabbit anti-mouse HRP,Abcam ab6728,RRID:AB_955440 Goat

(7) Sulconazole inhibits PD-1 expression in immune cells and cancer cells malignant phenotype through NF-κB and calcium activity repressionFrontiers in ImmunologyJanuary 5, 2024Simon Pernot, Mercedes Tomé, Isabel Galeano-Otero, Serge Evrard, Iker Badiola, Frederic Delom, Delphine Fessart, Tarik Smani, Geraldine Siegfried, Bruno O. Villoutreix, et al.TRPC1: rabbit anti-TRPC1 (1:500, T8276; Sigma-Aldrich, United States), STIM1: rabbit anti-STIM1 (1:500, 4916S; Cell Signaling, United States) and Orai1: rabbit anti-Orai1(1:250, O8264; Sigma-Aldrich, United States)

(8) Loss of STIM2 in colorectal cancer drives growth and metastasis through metabolic reprogramming and PERK-ATF4 endoplasmic reticulum stress pathwaybioRxivOctober 10, 2023Trayambak Pathak, J. Cory Benson, Martin T. Johnson, Ping Xin, Ahmed Emam Abdelnaby, Vonn Walter, Walter A. Koltun, Gregory S. Yochum, Nadine Hempel, Mohamed TrebakRRID:AB_331646" OXPHOS,Abcam,"Cat# ab110413, RRID:AB_2629281" STIM1,Cell Signaling Technology,Cat# 4916; RRID:AB_2271287 STIM2,Cell Signaling Technology,Cat# 4917; RRID:AB_2198021 IP3R1,Yule Lab,NA IP3R2,Yule

(9) Piezo1 induces endothelial responses to shear stress via soluble adenylyl Cyclase-IP(3)R2 circuitiScienceMay 19, 2023Santana Nunez D, Malik AB, Lee Q, Ahn SJ, Coctecon-Murillo A, Lazarko D, Levitan I, Mehta D, Komarova YA.AB_397704 anti-rabbit sAC,Thermo Fisher,PA5-25756; RRID: AB_2543256 anti-rabbit Stim1,Cell Signaling,4916; RRID: AB_2271287 anti-goat VE-cadherin,Santa Cruz,sc-6458; RRID: AB_2077955 anti-rabbit Total AKT,Cell Signaling

(10) SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING poolNature cell biologyMay 1, 2023Ji Y, Luo Y, Wu Y, Sun Y, Zhao L, Xue Z, Sun M, Wei X, He Z, Wu SA, et al.3294; Cell Signaling Technology), ERP44 (1:3,000; 2886; Cell Signaling Technology), STIM1 (1:2,000; 4916; Cell Signaling Technology), HA (1:2,000; H3663; Sigma–Aldrich), c-Myc (1:2,000; C3956; Sigma–Aldrich),

(11) Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replicationPLoS pathogensMarch 1, 2023Diao F, Jiang C, Sun Y, Gao Y, Bai J, Nauwynck H, Wang X, Yang Y, Jiang P, Liu X.rabbit anti-mTOR (Cell Signaling Technology, #2983), rabbit anti-STIM1 (Cell Signaling Technology, #4916), mouse anti-Orai1 (Proteintech, #66223–1), rabbit anti-GRP78 (Proteintech, #11587-1-AP) and rabbit

(12) CIC-39Na reverses the thrombocytopenia that characterizes tubular aggregate myopathyBlood advancesAugust 9, 2022Cordero-Sanchez C, Pessolano E, Riva B, Vismara M, Trivigno SMG, Clemente N, Aprile S, Ruffinatti FA, Portararo P, Filigheddu N, et al.dodecyl sulfate-polyacrylamide gel electrophoresis gels. The antibodies against STIM1 (Cell signaling, 4916S), ORAI1 (Invitrogen, PA1-74181), and Actin (Millipore, MAB1501) were prepared in TRIS-buffered saline

(13) Black Phosphorus Quantum Dots Enhance the Radiosensitivity of Human Renal Cell Carcinoma Cells through Inhibition of DNA-PKcs KinaseCellsMay 16, 2022Yue Lang, Xin Tian, Hai-Yue Dong, Xiang-Xiang Zhang, Lan Yu, Ming Li, Meng-Meng Gu, Dexuan Gao, Zeng-Fu ShangDNA-PKcs-ps2056 (Abcam, ab18192, 1:1000), Alexa-488-conjugated anti-rabbit secondary antibodies (CST, 4916, 1:1000), and Alexa-488-conjugated anti-mouse secondary antibodies (CST, 4408, 1:1000). 2.3. Flow Cytometry The

(14) STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cellseLifeFebruary 11, 2022Krishnan V, Ali S, Gonzales AL, Thakore P, Griffin CS, Yamasaki E, Alvarado MG, Johnson MT, Trebak M, Earley S.(Thermo Fisher Scientific) for 2 hr and incubated overnight at 4°C with primary antibody (anti-STIM1- [4916], Cell Signaling Technologies, Danvers, MA; anti-STIM1 [610954], BD Biosciences, Franklin Lakes, NJ;

(15) The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomainseLifeMay 11, 2021Zhemkov V, Ditlev JA, Lee WR, Wilson M, Liou J, Rosen MK, Bezprozvanny I.Biotechnology), anti-His tag (HIS.H8, 1:1000, Millipore), anti-calreticulin (ab2907, 1:5000, Abcam), anti-STIM1 (4916, 1:1000, Cell Signaling), and anti-IP3R1 (1:1000, produced in our lab). The HRP-conjugated anti-rabbit

(16) Omnitemporal choreographies of all five STIM/Orai and IP(3)Rs underlie the complexity of mammalian Ca(2+) signalingCell reportsMarch 2, 2021Emrich SM, Yoast RE, Xin P, Arige V, Wagner LE, Hempel N, Gill DL, Sneyd J, Yule DI, Trebak M.overnight at 4°C with primary antibody. The following antibodies and dilutions were used: STIM1 (1:2000; 4916, Cell Signaling Technology), STIM2 (1:1000; 4917, Cell Signaling Technology), α-tubulin (1:5000; 3873,

(17) BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammationThe Journal of clinical investigationNovember 2, 2020Volz J, Kusch C, Beck S, Popp M, Vögtle T, Meub M, Scheller I, Heil HS, Preu J, Schuhmann MK, et al.using anti-BIN2 antibody (Proteintech, 14245-1-AB), anti-STIM1 antibody (Cell Signaling Technology, 4916), anti-IP3R antibody (Merck-Millipore, 07-1210), anti-RhoA (Cytoskeleton, ARH04), and anti-GAPDH (MilliporeSigma,

(18) Store-operated Ca²⁺ entry in primary murine lung fibroblasts is independent of classical transient receptor potential (TRPC) channels and contributes to cell migrationScientific reportsApril 22, 2020Bendiks L, Geiger F, Gudermann T, Feske S, Dietrich A.dilutions: HRP-conjugated anti-β-actin antibody (Sigma A3854HRP, 1:10,000), anti-STIM1 (CellSignaling, #4916S, 1:1000), anti-STIM2 (CellSignaling, #4917S, 1:1000) and secondary anti-rabbit IgG peroxidase (POX)-antibody

(19) TRIC-A shapes oscillatory Ca2+ signals by interaction with STIM1/Orai1 complexesPLoS biologyApril 1, 2020Shrestha N, Bacsa B, Ong HL, Scheruebel S, Bischof H, Malli R, Ambudkar IS, Groschner K.BSA-TBST) were used: myc (1:1,000, 2276; Cell Signaling Technology, Danvers, MA, USA), STIM1 (1:1,000, 4916; Cell Signaling Technology), Orai1 (1:1,000, produced against C-terminal epitope ELAEFARLQDQLDHRGD and

(20) DeepBundle: Fiber Bundle Parcellation with Graph Convolution Neural NetworksGraph Learning in Medical ImagingNovember 14, 2019Feihong Liu, Jun Feng, Geng Chen, Ye Wu, Yoonmi Hong, Pew-Thian Yap, Dinggang Shen6,CA TP,4755,7726,4419,51967,30082,1330 FP,244,395,143,1100,602,0 FN,54,382,29,153,320,0 Counts,UF left,UF right,AF left,AF right,FX left,FX right TP,3535,4916,54448,42635,94,123 FP,0,0,199,495,6,7 FN,1,3,190,220,1,1 Classification results for 12 bundles

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11/19/2018STIM1 AntibodyDr. Mohammad AlamStromal interaction molecules (STIMs) function as Ca2+ sensors to detect changes in Ca2+ content in intracellular Ca2+ stores. STIM1 is a potential tumor suppressor; defects in STIM1 may cause ... Read Review

STIM1 Antibody

STIM1 Antibody from Cell Signaling Technology

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STIM1 Antibody from Cell Signaling Technology

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