The Best Mouse pDC-Specific Antibody

Immunology & Microbiology
Lewis Katz School of Medicine at Temple University
MD/PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fisher Scientific

Product Name:

Anti-Mouse CD317 (BST2, PDCA-1), PE-Cy7 clone: 3Bio927

Catalog Number:

501129098

Browse Similar Products: PDCA-1 Flow Cytometry Antibodies

This antibody reacts with PDCA-1 (also known as BST2, CD317), a membrane protein that is a specific marker of plasmacytoid dendritic cells (pDCs) in mice. I used this antibody at a 1:400 dilution to confirm my in vivo pDC depletion worked by staining the spleen with this antibody for analysis by flow cytometry. Samples were fixed after staining. The results from this antibody were beautiful! I also stained for CD11c, CD11b, B220, and Siglec H, and this antibody seems accurate, and the positive population was able to be identified very clearly. Highly recommend!

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen / plasmacytoid dendritic cells

Primary Incubation

Anti-PDCA-1 clone eBio927

Blocking Agent

PBS with 1% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Antibody appears to be both specific and bright

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to optimize, stains accurately and brightly

The Bad

None so far

The Bottom Line

Would highly recommend this clone for a pDC-specific stain for flow.

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