Santa Cruz
Rab 34 antibody (C-5)
sc-376710
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I am interested in studying the molecular mechanism of hedgehog signal transduction in vertebrate cells. I have performed a CRISPR/Cas9 genetic screen to uncover new regulators of hedgehog signaling pathway. One of the positive regulators identified in my screens was Rab34. In this experiment, I had knocked out Rab34 in NIH-3T3 cells using lentiCRISPR v2 strategy. The result is a pooled knockout cell line for Rab34. Rab34 antibody from Santa Cruz picked up a band at ~29 kDa. The predicted size is 29 kDa.
Western Blot
NIH-3T3 whole-cell extract
1:500 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% milk in TBS-T
1:10,000 dilution of donkey anti-mouse IgG+HRP; 1 hr at room temperature
None
ECL regular
Picks up a band at 29 kDa and the signal is strong in NIH-3T3 cells.
N/A
Specific band at 29 kDa
May be one more non-specific band (could also be another isoform)
Good antibody for western blotting