Thermo Fisher Scientific
Dynabeads® Human T-Activator CD3/CD28 for T Cell Expansion and Activation
11132D
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We used these beads for activation and expansion of human CD8 and CD4 T cells. These beads lead to robust activation of T cells.
Activation and expansion of human T cells
Purified naive human CD4 or CD8 T cells
Beads are washed with appropriate media using a magnet. The beads are added to cell culture 1 µl of beads per 10^4 cells. Examine the cultures daily for size, shape, and proliferation. Clustering should occur when cells are attached to beads.
Make sure to wash beads since they contain azide.
Cells are robustly activated and expanded. Cells are fully functional compared to naive T cells.
N/A
Easy-to-use way to activate T cells. Bead stimulation also leads to consistent activation between experiments.
Expensive
These beads work great for activation and expansion of human T cells.