LC3B Antibody 2775S from Cell Signaling Technology

Product Specs
ItemLC3B Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2775S
Quantity100 µl
ApplicationsW
ReactivityH, M, R
TargetLC3B
HostRabbit
NCBI Gene AliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
NCBI Full Gene Namemicrotubule associated protein 1 light chain 3 beta
ClonalityPolyclonal
Research AreaAutophagy
Molecular Weight14, 16

Questions and Answers

Can this antibody be used to study autophagy in cancer models?

Yes, the LC3B antibody has been shown to be effective in studying autophagy in various cancer models. For instance, it was utilized in the characterization of autophagy-related proteins in studies involving HER2+ breast cancer, where autophagy was linked to tumorigenesis and cell survival mechanisms ().

Is the LC3B antibody suitable for use in immunoblotting applications?

The LC3B antibody has been successfully employed in immunoblotting, as evidenced by its use in multiple studies to detect LC3B protein levels, which are critical for assessing autophagic flux (, ).

What species can this antibody react with, and is it effective for human samples?

The LC3B antibody is reactive with mouse, rat, and human samples, making it suitable for studies involving these species. Its effectiveness in detecting endogenous levels of LC3B protein in human samples has been documented in various research contexts (, ).

Are there any specific experimental conditions or treatments that enhance the detection of LC3B using this antibody?

Yes, treatments that induce autophagy, such as exposure to palmitic acid or the use of autophagy inhibitors like 3-MA, have been shown to affect LC3B levels. For example, palmitic acid treatment increased LC3-II expression in hepatocyte models, which can be detected using the LC3B antibody ().

Cell Signaling Technology

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United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Bilirubin reduces mortality in sepsis models by inhibiting NOX2-mediated formation of neutrophil extracellular trapsRedox RepDecember 31, 2026Sang-Jin Kim, Chang Woo Ko, Wan-Seo Kim, Yeon Jun Kang, Chul-Hwan Lee, Won-Woo Lee, Jong-Wan Park(Irvine, CA); NADPH (481973) from Calbiochem (Burlington, MA). Antibodies against H3 (3638, 1:5000), LC3B (2775, 1:2000), Rab7 (2094S, 1:1000) and GAPDH (2118, 1:5000) were purchased from Cell Signaling Technology

(2) Ultrasound-triggered platelet vehicles loading fluorescein for targeted sonodynamic therapy of glioblastomaDrug DelivDecember 31, 2026Yu-Xin Guo, Qing Deng, Ke Li, Quan Zhang, Jie Guo, Meng-Fei Wang, Jia-Lin Wu, Hui-Mei Lei, Yue-Ying Chen, Huan Pu, et al.(65050-1-Ig) from Proteintech; γ-H2AX (AP0687) and SMUG 1 (A10166) from Abclonal; Caspase 3 (14220S) and LC3B (2775S) from Cell Signaling Technology; p-ATR (GTX132146, GeneTex); HIF-1α (20960-1-AP, Proteintech); and LIG

(3) Global and mitochondrial choline dehydrogenase contribute to goose fatty liver formation by differentially regulating energy metabolism, autophagy and oxidative stress related pathwaysPoultry ScienceOctober 1, 2026Xiaoyi Zhou, Mengqing Lv, Ya Xing, Xiangli Zhao, Meiyi Mao, Minmeng Zhao, Daoqing Gong, Chuanju Liu, Tuoyu Geng, Jing GeCell Signaling Technology, USA), Bax (50599-2-Ig, Proteintech, China), SOD2 (24127-1-AP, Proteintech, China), LC3B (2775, Cell Signaling Technology, USA), Hsp90 (60318-1-Ig, Proteintech, China), CHDH (sc-393885, Santa Cruz Biotechnology

(4) Dynamic cardiac hyperplasia and hypertrophy in Burmese PythonsAmerican Journal of Physiology-Cell PhysiologySeptember 1, 2026Yuxiao Tan, Thomas G. Martin, Angela K. Peter, Christopher D. Ozeroff, Christopher C. Ebmeier, Ryan Doptis, Brooke Harrison, Leslie A. LeinwandBSA/0.2% TWEEN-20/TBS at 4°C overnight using the following dilutions: LC3B (Cell Signaling Technology, 2775), 1:1000. Membranes were subsequently washed with TBS three times (5 min for each wash), and incubated

(5) Transglutaminase 2 regulates vimentin-dependent proteostasis during macrophage activationSci AdvAugust 28, 2026Yali Xu, Ting Su, Hricha Mishra, Naoshi Dohmae, Takehiro Suzuki, Yuriko Sakamaki, Haruyo Aoyagi, Hideki Aizaki, Hajime Nishimura, Erina Furuhata, et al.anti-ubiquitin (1:1000, Santa Cruz Biotechnology sc-8017), anti-LC3B (1:1000, Cell Signaling Technology 2775S), anti-p62 (1:1000, Santa Cruz Biotechnology sc-48402), anti-Traf6 (1:1000, Cell Signaling Technology

(6) Autophagic responses to vigorous endurance exercise in men vary by training status but are similar in PBMCs and skeletal muscle: A pilot studyPhysiol RepAugust 19, 2026Jonathan W. Specht, Jeremy B. Ducharme, Alyssa R. Bailly, Michael R. DeyhleNo. 5114, Cell Signaling Technology, RRID:AB_10624872) and rabbit polyclonal LC3B (1:1000, Cat. No. 2775, Cell Signaling Technology, RRID:AB_915950). Following overnight incubation, membranes were washed with

(7) c-Fos enhances influenza virus replication by stabilizing the M2 protein and promoting autophagosome accumulationJournal of VirologyAugust 18, 2026Xiaonan Chen, Junyang Yan, Mengxue Li, Hanbin Liu, Daiqiang Lu, Yang Wang, Yun Liu, Qiao Zhang, Feng Gao, Jiaojiao Pengshort interfering RNA (siRNA) oligonucleotides The following primary antibodies were used: anti-LC3B (2775; Cell Signaling Technology, CST), anti-SQSTM1 (5114; CST), anti-GAPDH (2118; CST), anti-NP (10780-01;

(8) Chronic Hypoxia-Induced Reprogramming of Smooth Muscle Cells: Insights into Transcription Factors, Cellular Stresses, and Therapeutic ImplicationsBiomedicinesAugust 16, 2026Sheng-Huei Wang, Yuan-Ming Tsai, Shu-Ting Liu, Shih-Ming Huang(catalog no.: 4695, 1:2000 dilution), H3 (catalog no.: #9715, 1:3000 dilution), LC3B (catalog no.: #2775, 1:1000 dilution), and p-NFκB-p65 (S536) (catalog no.: 3033, 1:1000 dilution) were obtained from Cell Signaling Technology

(9) Intranasal Administration of Insulin and IGF-1 Protects Hippocampal CA1 Neurons Against Transient Global Forebrain Ischemia by Inhibiting Autophagy, Apoptosis and Neuroinflammation: A Comparative StudyInt J Mol SciAugust 11, 2026Irina O. Zakharova, Liubov V. Bayunova, Daria K. Avrova, Natalia F. Avrovamanufacturer’s recommendations. The following primary antibodies were used: anti-LC3B-I/II (1:1000, #2775, Cell Signaling, Danvers, MA, USA), anti-IL-6 (1:1000, #DF6087, Affinity Biosciences, Changzhou, China),

(10) A double-duty champion: Eurycoma longifolia counters oxidative stress and unlocks autophagy to safeguard neurons and muscle cellsPLoS OneAugust 10, 2026Qing Huang, Shinong Gu, Hengzhi Deng, Annie George, Ashril Yusoftemperature. Primary antibodies against LC3B (rabbit monoclonal, 1:200; Cell Signaling Technology, USA, #2775) and p62 (mouse monoclonal, 1:200; Cell Signaling Technology, USA, 39749S) were applied overnight at

(11) FLCN loss is characterized by SQSTM1/p62 accumulation despite functional autophagy flux in Birt-Hogg-Dubé syndrome-associated kidney cancerAutophagy RepAugust 8, 2026Halvor Ullern, Julie Aarmo Johannessen, Feyza Kasikci, Miriam Formica, Naghmeh Karimi Melve, Siri Andresen, Andreas Brech, Karol Axcrona, Kjersti Jørgensen, Lorant Farkas, et al.(Cell Signaling Technologies, 3697 – 1:1000 WB), LC3 (MBL, PM036 – 1:500 IF), LC3 (Cell Signaling Technologies, 2775 – 1:1000 WB), phospho-RPS6K Thr389 (Cell Signaling Technologies, 9205 – 1:1000 WB), phospho-TFEB Ser211

(12) Loss of Rab8a-Dependent Tethering of Lipid Droplets to Mitochondria Contributes to Hypoxia-Reoxygenation InjuryMedComm (2020)August 6, 2026Jialing Tang, Nathan Joyce, Thomas Laval, Eddie Tam, Hye Kyoung Sung, Mireille Ouimet, Gary Sweeney(BD Science; 610844), PLIN2 (Proteintech; 60340), PLIN5 (Proteintech; 26951), LC3 (Cell Signaling; 2775), p62 (Cell Signaling; 5114), HIF1⍺ (Cell Signaling; 79233), CPT1⍺ (Proteintech; 15184‐1‐AP), Tom20

(13) Nanoplastics Impair Neuroimmune Integrity Via Cellular Retention and Multiple Organelle StressJ Hazard Mater AdvAugust 6, 2026Chman Shahzadi, Pathik Parekh, Buyandelger Batsaikhan, David Tweedie, Erica Costantini, Elliot J Glotfelty, Piero Di Carlo, Marcella Reale, Nigel H. Greigunlabeled NPs (100, 200, or 1000nm). This involved primary antibody LC3B (Cell Signaling Technology, Cat# 2775S; 1:100) and LAMP1 (Invitrogen, Cat# MA1–164 ;10 μg/mL) 24h incubation at 4 °C, followed by secondary

(14) Cannabidiol-induced Heme oxygenase-1 contributes to modulate the phenotype of hiPSC-derived cardiac fibroblasts from patients with Duchenne muscular dystrophyRedox BiolAugust 5, 2026L. Savchenko, S. Soussi, D. Rovina, A. Swiader, S. Mallia, G. Pompilio, O. Kunduzova, A. Parini, A. Gowran, N. PizzinatHO -1,Proteintech,10701-1-AP Hsp90 (4F10),Santa Cruz Biotechnology,sc-69703 LC3B Antibody,Cell Signaling Technology,#2775 LDHA,Santa Cruz Biotechnology,sc-137243 PDHE1a,Santa Cruz Biotechnology,sc-377092 Phospho PDHE1a,Cell Signaling Technology

(15) GSH-Responsive Co-Delivery of Chrysin and 3‑Methyladenine Disrupts Tumor Adaptive Stress for Synergistic Antitumor TherapyACS OmegaJuly 21, 2026Wenbin Kong, Baoqiang Su, Han Gu, Weiqiang Huang, Jing Wang, Yaqi Zhu, Rongrong Xu, Dali Tong, Weiping XuVEGF (cat. no. YM8345) were purchased from Immunoway (Plano, TX, USA). Antibody against LC-3 (cat. no. 2775S) was purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against p62 (cat. no. ab109012)

(16) Dual action of pimozide through lysosome disruption and inhibition of STATs induces apoptosis in chronic lymphocytic leukemia (CLL)Blood AdvancesJuly 14, 2026Madhumita S. Manivannan, Madhumita S Manivannan, Xiaoyan Patel, Anthea Peters, Spencer B. Gibson14025092), and 7-AAD (BD Biosciences; 559925). Primary antibodies used include LC3B (Cell Signaling; 2775S), β-actin (Sigma; A5441), α-tubulin (Cell Signaling; 2125), PARP (Cell Signaling; 9542), cathepsin D

(17) Targeting Phosphatidylserine Synthesis for Tumor Cell Suppression in Esophageal Squamous Cell Carcinoma and GlioblastomaInt J Mol SciJuly 13, 2026Yixuan Hu, Yaqi Cui, Zeqiong Xu, Duo Wu, Xiaochun Yu1 h blocking step with 5% BSA, samples were incubated with anti-LC3B primary antibody (1:200; CST, #2775) overnight at 4 °C. Cells were then stained with an Alexa Fluor 488-conjugated secondary antibody (1:500)

(18) Investigating the Mechanisms Underlying Cell Death Induction by a Tributyltin Molecule in HTLV-1-Infected Cells Dependent or Not on IL-2 as a Growth FactorInt J Mol SciJuly 10, 2026Valeria Stefanizzi, Evariste Molimbou, Emanuela Balestrieri, Antonella Minutolo, Franca M. Cordero, Sandro Grelli, Antonio Mastino, Claudia Matteucci, Beatrice Macchi, Francesca Marino-MerloProteintech, Chicago, IL, USA), anti-caspase-8 (#AF1650, R&D Systems, Minneapolis, MN, USA), anti-LC3 (#2775S, Cell Signaling Technology), anti-GSDMD (#97558, Cell Signaling Technology), and anti-β-actin (#66009-1,

(19) Effects of empagliflozin alone and in combination with exercise on the soleus muscle in obese type 2 diabetic ratsJ Physiol SciJuly 7, 2026Kazuho Inoue, Saori Sekiguchi, Yuji Ogura, Seiko Hoshino, Kimie Katayama, Junko Asano, Takayuki Akagi, Junko Igarashi-Migitaka, Shiika Watanabe, Yoshio Nagai, et al.(COX5B, 1:10,000; ab180136; Abcam), microtubule-associated protein 1 light chain 3 beta (LC3B, 1:1000; #2775; Cell Signaling Technology, Danvers, MA, USA), phospho-Unc−51-like kinase 1 (Ser317) (p-ULK1, 1:1000;

(20) CRISPR screening identifies TRIM27 as a destabilizer of the Smith–Magenis syndrome protein RAI1GENETICSJuly 6, 2026Yu Cheng Lin, Yu-Ju Lee, Catherine Xinrui Li, Hao-Cheng Chang, Max Kowalczyk, Minza Haque, Adrian Dragoiescu, Truc T Losier, James A Taylor, Maxime W C Rousseaux, et al.anti-TRIM27 (1:1,000; Cell Signaling Technology 15099S), rabbit anti-LC3B (1:1,000; Cell Signaling Technology 2775S), mouse anti-HA (1:1,000; Abcam ab18181), rabbit anti-HA (1:1,000; Cell Signaling Technology 3724S),

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(1)

10/27/2025Reliable Antibody for Autophagy Analysis in Lung Homogenates Edma LokuI measured autophagy levels in lung homogenates from mice. The antibody worked effectively and was employed in Western blotting using Simple Western technology.
(1)

12/16/2022LC3 Antibody Works Well Mikyoung You25mg mouse liver lysate was applied into 10% SDS-page and incubated with LC3 antibody (1:1,000, in 5% BSA) overnight.
(1)

3/1/2019Product Worked Perfectly to Illuminate Autophagy by IF Mark EAntibody performs very well and comes at a very good price. 1:500 yielded good signal.
(1)

10/19/2018Good LC3 Antibody for Western Blot and Immunofluorescence Wei Yang SitUsed the LC3 antibody to carry out Autophagy studies in the cells and as well as immunofluorescence studies. In summary, we are able to see how the protein expression level changes in respond to ... Read Review
(1)

9/16/2016LC3B Antibody Ramanath HegdeUpon induction of autophagy LC3B-I is converted to LC3B-II through lipidation. This conversion is used to indicate the induction of autophagy under various conditions like starvation or treatments. ... Read Review

LC3B Antibody

LC3B Antibody from Cell Signaling Technology

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LC3B Antibody from Cell Signaling Technology

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