Good H3K9me3 Antibody for Western Blots

October 05, 2018

BlueskyReddit
School of Graduate Studies
SUNY Downstate Medical Center
Graduate Student

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Company:

Cell Signaling Technologies

Product Name:

Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb

Catalog Number:

13969T

Our aim was to determine changes in methylation pattern of H3K9me3 by changing Setdb1 expression in PC3 cells. A good antibody for epigenetics study.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines PC3

Primary Incubation

Overnight, 4 degree C, 1:1000

Blocking Agent

5% milk

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

N/A

Detection

hrp-dab reaction

Results Summary

H3K9me3 was expressed in cell lines, however, changes in methylation pattern were not observed by changing the expression of Setdb1. H3 was used as a control to determine changes in H3K9me3 expression.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Highly specific, no cross reactivity

The Bad

None

The Bottom Line

Good antibody for epigenetics study

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