Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb 13969T from Cell Signaling Technology

Product Specs
ItemTri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb
CompanyCell Signaling Technology
Price $163.00 Inquire
Catalog Number13969T
Quantity20 µl
Molecular Weight17
Research AreaChromatin Regulation / Nuclear Function
ReactivityHu, Ms, Rt, Mk
ClonalityMonoclonal
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
NCBI Full Gene NameH3 clustered histone 1
ApplicationsWB, FCM, ICC, IF, IP, ChIP
HostRabbit
TargetH3 (Lys9) trimethylate

Questions and Answers

Can this antibody be used to study the role of H3K9me3 in cancer research?

Yes, the Tri-Methyl-Histone H3 (Lys9) antibody has been utilized in various studies related to cancer. For instance, it was used in research demonstrating that SPIN1 promotes chemoresistance and homologous recombination repair by enhancing the binding of Tip60 to H3K9me3, indicating its relevance in cancer biology and treatment responses ().

Is this antibody effective for immunofluorescence applications?

The Tri-Methyl-Histone H3 (Lys9) antibody has been shown to work effectively in immunofluorescence applications. For example, it was used in a study where primary antibodies were applied to cells for immunofluorescence staining, confirming its utility in visualizing histone modifications in cellular contexts ().

What species can this antibody be used with?

This antibody is reactive with multiple species, including humans, mice, rats, and non-human primates, making it versatile for various experimental models in cell biology and epigenetics.

Does this antibody cross-react with other histone modifications?

The Tri-Methyl-Histone H3 (Lys9) antibody specifically detects tri-methylated histone H3 at Lys9 and shows some cross-reactivity with di-methylated histone H3 at the same position. However, it does not cross-react with non-methylated or mono-methylated histone H3 Lys9, nor with other methylated histones such as H3K4, H3K27, H3K36, or H3K79.

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Genome instability is increased during DNA replication in the presence of the CPD UV photoproduct in transcriptionally active genesiScienceJuly 17, 2026Rika Kusumoto-Matsuo, Yusuke Matsuno, Naoki Akiba, Yuya Manaka, Jiro Toshima, Fumio Hanaoka, Ken-Ichi Yoshiokaab5176 HP1α,Cell Signaling Technology,Cat# 2616 tri-methyl H3 (Lys 9),Cell Signaling Technology,Cat# 13969 Acetyl H3 (Lys 9),Abcam,Cat# ab10812 phospho RPA32 (Ser 33),Novus Biologicals,Cat# NB100-544 mouse

(2) ZNF274 constrains lineage plasticity and drives intrinsic resistance to CDK7 inhibitors in pancreatic cancerNature CommunicationsMay 23, 2026Jessica E. Gianopulos, Aidan Schutter, Stephanie Dobersch, Adrianne Wallace-Povirk, Sophie E. Kogut, Andrea Doak, Pritha Chanana, Sabrina Ge, Luke Mangino, Naomi Yamamoto, et al.Antibodies used were anti-ZNF274 (1:500, Novus Biologicals #H00010782-A01), anti-H3K9me3 (1:1000, CST #13969), anti-ZEB1 (1:500, abcam #ab203829), anti-EpCAM (1:1000, abcam #ab32392), anti-E-cadherin (1:1000,

(3) Mitochondrial l-2-hydroxyglutarate is a physiological signalling metaboliteNatureMay 20, 2026Ram P. Chakrabarty, Jonathan G. Van Vranken, Yuki Aoi, Taylor A. Poor, Gregory S. McElroy, Karthik Vasan, Shimaa H. A. Soliman, Marta Iwanaszko, Rogan A. Grant, Benjamin C. Howard, et al.pulldown of chromatin was carried out overnight at 4 °C using the following antibodies: H3K9me3 (CST, 13969) and H3K27me3 (CST, 9733S). Drosophila spike-in chromatin and antibody were added as an internal normalization

(4) Obesity Disrupts H3K4me3-Mediated Lactate Accumulation and Efferocytosis in Hypoxic MacrophagesFASEB JApril 15, 2026Kentaro Takahashi, Julia Drolet, Jinghua Liu, Jasmine R. Jackson, Madison Babcock, Muthusamy Thiruppathi, Milie Fang, Tyler Paul, Gayathri Ganesh, Yuri Fukasawa, et al.Cell Signaling), Arg1 (GTX109242, GeneTex), β‐actin (3700, Cell Signaling), H3K4me3 (ab8580, Abcam), H3K9me3 (13969, Cell Signaling), H3K27me3 (9733, Cell Signaling), H3K18la (PTM‐1406, PTM BIOLABS), and total H3 (3638,

(5) Antigenic cancer persister cells survive direct T cell attackbioRxivApril 12, 2026Michael X. Wang, Brandon E. Mauch, August F. Williams, Tania Barazande-Pour, Filipe Araujo Araujo Hoffmann, Sophie H. Harris, Cooper P. Lathrop, Claire E. Turkal, Bryan S. Yung, Michelle H. Paw, et al.SLC7A11 (CST #12691, 1:1000), H3K14ac (CST #7627, 1:1000), H3K4me3 (CST #9751, 1:1000), H3K9me3 (CST #13969, 1:1000), H3K27me3 (CST #9733T, 1:1000), Histone H3 (CST #3638, 1:1000), MART-1 (CST #34511, 1:1000),

(6) BRD4 directs myofiber identity and metabolic adaptation through CHD4 cooperationNature CommunicationsApril 9, 2026Zheng Zhou, Lin Liu, Zhisheng Xu, Danxia Zhou, Anqi Liu, Jiachen Yuan, Gonghao Shen, Qiqi Guo, Yujing Yin, Yan Mao, et al.(catalog 14186, 1:1000 dilution), anti-H3K9me2 (catalog 4658, 1:1000 dilution), anti-H3K9me3 (catalog 13969, 1:1000 dilution), anti-H3K36me3 (catalog 4909, 1:1000 dilution), anti-H2A (catalog 12349, 1:1000 dilution)

(7) Obesity Disrupts H3K4me3‐Mediated Lactate Accumulation and Efferocytosis in Hypoxic MacrophagesFASEB JMarch 29, 2026Kentaro Takahashi, Julia Drolet, Jinghua Liu, Jasmine R. Jackson, Madison Babcock, Muthusamy Thiruppathi, Milie Fang, Tyler Paul, Gayathri Ganesh, Yuri Fukasawa, et al.Cell Signaling), Arg1 (GTX109242, GeneTex), β‐actin (3700, Cell Signaling), H3K4me3 (ab8580, Abcam), H3K9me3 (13969, Cell Signaling), H3K27me3 (9733, Cell Signaling), H3K18la (PTM‐1406, PTM BIOLABS), and total H3 (3638,

(8) Hypoxic regulation of chromatin and gene transcriptionCommunications BiologyMarch 25, 2026Jessica D. Kindrick, Olivia Lombardi, Silvia Halim, Veronique N. Lafleur, Cindy H. Chau, William D. Figg, Peter J. Ratcliffe, David R. Mole1:1000), anti-H3K4me3 (Cell Signaling, cat no. 9751S, 1:3000), anti-H3K9me3 (Cell Signaling, cat no. 13969S, 1:3000), anti-H3K27me3 (Cell Signaling, cat no. 9733S, 1:3000), anti-H3K36me3 (Cell Signaling, cat

(9) Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genesMol CellMarch 19, 2026Steven Ingersoll, Abby Trouth, J. Carlos Angel, Xinlong Luo, Axel Espinoza, Joey Wen, Chengjie Zhu, Joseph Tucker, Kalkidan Astatike, Christopher J. Phiel, et al.AB_2235915" Anti-H3K27Me3,Cell Signaling,"Cat# 9733S, RRID: AB_2616029" Anti-H3K9Me3,Cell Signaling,"Cat# 13969, RRID: AB_2798355" Alexa Fluor 488-Goat anti-Mouse,Life Technologies,"Cat# A11029, RRID: AB_2534088" Alexa

(10) Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D☆Bioact MaterMarch 19, 2026Sarah J. Loveland, Xinming Tong, Manish Ayushman, Hung-Pang Lee, Julia M. Johannsen, Callie M. Weber, Jake Song, Michelle Tai, Georgios Mikos, Yara Chaib, et al.antibodies and dilutions are listed as follows: anti-Lamin A/C (CST 2032, 1:1000), anti-H3K9Me3 (CST 13969, 1:1000), anti-integrin beta 1 (CST, 1:1000), anti-GAPDH (CST 5174, 1:10000), and goat anti-rabbit IgG

(11) Maternal obesity disrupts epigenetic reprogramming via peroxisomal-dependent phospholipid-methyl uncoupling during zygotic genome activationNature CommunicationsMarch 18, 2026Xiao-Guohui Zhang, Lin-Li Yang, Xie Feng, Yu-Wei Zhang, Lei Guo, Sun-Xing Huang, Tie-Gang Meng, Pei-Ying Li, Lei-Ning Chen, Rui-Bao Su, et al.PMP70 (ab109448, Abcam); PEX5 (HPA039259,Merck); H3K4me3 (9751S, Cell Signaling Technology); H3K9me3 (13969T, Cell Signaling Technology); H3K27me3 (K003334P, Solarbio); H3K27ac (K005114P, Solarbio); H3K9ac (K005116P,

(12) NPM1 mislocalization mediated by RNA Pol I inhibition alters chromatin landscapebioRxivFebruary 23, 2026Pierre-Olivier Estève, Sagnik Sen, Karthikeyan Raman, Ashwin Unnikrishnan, Sriharsa Pradhansubsequent SUV39H1 assay, Western blot was performed using anti-H3K9ac (CST # 9649S) and anti-H3K9me3 (CST # 13969S). Anti-histone H3 (CST # 4499S) was used as control. The percentage of histone H3 acetylation and methylation

(13) MYSM1-mediated epigenetic modification dysregulation leads to immunosuppression and secondary infections in sepsisPLOS PathogensFebruary 12, 2026Jiali Xiong, Xin Cheng, Xiaoxing Xiong, Heyang Zhang, Qi An, Zhiqiang Li, Hong Fan, Guangli Li, Wei Li, Mingfu Tian, et al.(Cambridge, UK). Anti-H2AK119ub (8240S), anti-H3K36me3 (4909S), anti-H3K79me3 (4260S), anti-H3K9me3 (13969T), anti-H3K27me3 (9733T), anti-H3K27ac (8173T), and anti-H2A (12349S) were purchased from Cell Signaling Technology

(14) Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegenerationCell Stem CellFebruary 5, 2026Nathalie Saurat, Andrew P. Minotti, Maliha T. Rahman, Trisha Sikder, Chao Zhang, Daniela Cornacchia, Johannes Jungverdorben, Gabriele Ciceri, Doron Betel, Lorenz Studeranti Lap2,BD Bioscience,Cat# 611000 RRID: AB_398313 Rabbit anti H3k9me3-PE,Cell Signaling Tech,Cat# 13969S RRID: AB_2798355 "Anti-mouse IgG, HRP-linked",Cell Signaling Tech,Cat#7076S RRID: AB_330924 "Anti-rabbit IgG, HRP-linked

(15) ZFTA–RELA ependymomas make itaconate to epigenetically drive fusion expressionNatureFebruary 4, 2026Siva Kumar Natarajan, Joanna Lum, James Haggerty Skeans, Minal Nenwani, Sanjana Eyunni, Mateus Mota, Jill M. Bayliss, Akash Deogharkar, Erin Taya Hamanishi, Matthew Pun, et al.5676, 1:1,000); H3K4me3 (Cell Signaling Technology, 9751, 1:1,000); H3K9me3 (Cell Signaling Technology, 13969, 1:1,000); H3K27Ac (Cell Signaling Technology, 8173, 1:1,000); H3K27me3 (EMD Millipore, 07-449, 1:1,000);

(16) DNA damage induced by HIV-1 Vpr triggers epigenetic remodeling and transcriptional programs to enhance virus transcription and latency reactivationPLoS BiolFebruary 2, 2026Nicholas Saladino, Emily Leavitt, Hoi Tong Wong, Jae-Hoon Ji, Diako Ebrahimi, Daniel J. Salamangoacetyl-histone H3K9 (1:1,000, Cell Signaling 9649), tri-methyl-histone H3K9 (1:1,000, Cell Signaling 13969), acetyl-histone H3K14 (1:1,000, Cell Signaling 7627), acetyl-histone H3K18 (1:1,000, Cell Signaling

(17) Fork Reversal Safeguards Epigenetic Inheritance During Replication StressResearch SquareJanuary 29, 2026Wenpeng Liu, Caixian Zhou, Caixian Zhou, Nadia I. Martin, Nadia Martin, Tae-Hee Gwit, Tae-Hee Hedglin, Roséa Hedglin, Roséa Chen, Tianpeng Zhang, et al.dilution), H3 (Abcam ab1791) (1:200 dilution), H3K9ac (Millipore 07-352) (1:100 dilution), H3K9me3 (CST 13969) (1:100 dilution), H4 (CST 13919) (1:100 dilution); mouse biotin (Millipore B7653) (1:200 dilution)

(18) Metabolic and epigenetic abnormalities cause hepatic fibrogenesis in metabolic dysfunction–associated steatohepatitis model miceJournal of Biological ChemistryJanuary 1, 2026Atsushi Miura, Shiori Ikeda, Yuki Kono, Keigo Kawate, Takashi Hosono, Taiichiro Sekidilution, catalog no.: 9751; Cell Signaling Technology), rabbit anti-H3K9me3 (1:1000 dilution, catalog no.: 13969; Cell Signaling Technology), rabbit anti-H3K27me3 (1:1000 dilution, catalog no.: 9733; Cell Signaling Technology

(19) Hyperviscous Diabetic Bone Marrow Niche Impairs BMSCs Osteogenesis via TRPV2‐Mediated Cytoskeletal‐Nuclear MechanotransductionAdvanced ScienceDecember 22, 2025Yao Wen, Xinhui Zheng, Jieliu Li, Minyu He, Dongqi Fan, Xingyu Zhu, Qiming Zhai, Liangjing Xin, Tao Chenusing Qubit. The library was then constructed. The antibodies used in the ChIP assay were H3K9me3 (#13969T, CST, 4 µg test−1). 4.25 Abaqus Simulation The influence of medium viscosity on cell confining stress

(20) Diet-induced RKIP downregulation disrupts PC/PE-ER homeostasis to drive MASLDNat CommunDecember 12, 2025Mengjie Li, Qian Ou, Qiang Qin, Jiaxi Chen, Sen Yang, Jie Zhao, Hua Meng, Xu Li, Pinglong Xu, Cunqi Ye, et al.ABclonal, A12768, ARC0704), anti-GAPDH (1:2000, Proteintech, 60004-1-Ig, 1E6D9), anti-H3K9me3 (CST, 13969, D4W1U), anti-H3K27me3 (CST, 9733, C36B11), anti-H3K4me3 (CST, 9751, C42D8), anti-H3K36me3 (CST, 4909,

Write a Review

(1)

10/5/2018Good H3K9me3 Antibody for Western BlotsMs. Kaveri AroraOur aim was to determine changes in methylation pattern of H3K9me3 by changing Setdb1 expression in PC3 cells.

Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (193)

Reviews

Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map