Anti-HCN1 OK for Cell Immunofluorescence

October 08, 2018

Biomedical Sciences
Humanitas Clinical and Research Center
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Alomone Labs

Product Name:

Anti-HCN1 Antibody

Catalog Number:

APC-056

I used the antibody to assess the localisation of HCN1 48 hours after its transient transfection in HEK293. In summary, this anti-HCN1 antibody specifically stains HCN1-transfected cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

HEK 293 cell line

Primary Incubation

1:75, 1 hour, 23°C

Blocking Agent

BSA 2%

Secondary Incubation

50 minutes, 23°C

Tertiary Incubation

N/A

Detection

Confocal microscope

Results Summary

The anti-HCN1 antibody specifically stains HCN1-transfected cells, with low aspecific background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specifically stains HCN1

The Bad

Need more optimisation at lower concentrations

The Bottom Line

Ok for immunofluorescence on PFA fixed cells.

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