Great Myosin VIIa Antibody for Immunofluorescence

October 03, 2018

Biomedical Sciences
Humanitas Clinical and Research Center
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa-Cruz Biotechnology

Product Name:

Myosin VIIa Antibody (C-5)

Catalog Number:

sc-74516

I used the anti-myosin VIIa antibody to visualise the sensory cells in a mouse whole-mount cochlea. In summary, this antibody performed well on PFA-fixed whole-mount cochlea.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse cochlea

Primary Incubation

1:300, overnight, 23°C

Blocking Agent

10% normal horse serum in 0.5% Tween in PBS

Secondary Incubation

50 minutes, 23°C

Tertiary Incubation

N/A

Detection

Confocal microscope

Results Summary

The antibody performed well on PFA-fixed whole-mount cochlea, clearly staining the sensory epithelium.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works in immunofluorescence on tissue

The Bad

Nothing

The Bottom Line

Recommended as inner ear hair cell marker.

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