Nice CD56 Antibody for NK Cells

Innate Immunity
Deutsches Rheuma-Forschungszentrum (DRFZ)
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD56 (NCAM) Antibody PE-Dazzle594

Catalog Number:

318348

The CD56 antibody in PE-Dazzle594 from Biolegend is a good alternative with a bright staining to leave more common fluorochromes open for less common and less bright antigens. Its resolution is good enough to distinguish CD56 bright and dim Natural Killer cell subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

1:200, 15 min at RT

Blocking Agent

none

Secondary Incubation

-

Tertiary Incubation

-

Detection

Flow Cytometry on an LSR Fortessa II

Results Summary

Bright staining with a good resolution

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright staining, more common fluorochromes like PE and APC can be used for less common antigens

The Bad

Compensation in PE generates a bit of a spreading error (esp. in the CD56bright)

The Bottom Line

Nice antibody

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