Anti-CD31 for Paraffin Embedded Samples

September 20, 2018

BlueskyReddit
Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-CD31

Catalog Number:

ab28364

This antibody was used to monitor angiogenesis during wound healing in murine full excision skin wounds. Samples were taken at multiple time points and endothelial cells were stained using this CD31 antibody.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse skin

Primary Incubation

1:50

Blocking Agent

5%NGS / 1xPBS-0.1%TritonX100 at RT for 60 minutes

Secondary Incubation

Rabbit biotinylated antibody 1:200

Tertiary Incubation

-

Detection

DAB Kit (Vector Cat SK-4100)

Results Summary

This antibody was used to achieve clean staining of blood vessels in mouse skin

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean staining

The Bad

Requires an antigen unmasking step

The Bottom Line

A good antibody to detect Cd31 in paraffin embedded samples

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