Cell Count and Viability Using the LUNA-FL

September 14, 2018

BlueskyReddit
University of Helsinki
Institute for Molecular Medicine Finland FIMM
Postdoctoral Researcher

Overall

Performance

Ease-of-Optimization

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Company:

Logos Biosystems

Product Name:

LUNA-FL™ Dual Fluorescence Cell Counter

Catalog Number:

L20001

We use LUNA-FL for counting and measuring cell viabilities prior submitting the cells to single cell RNA-seq analysis. LUNA-FL was chosen because of it's good capability to count cells and also determine the cell viabilities. Additinally image and report export is easy with LUNA-FL.

Experimental Design and Results Summary

Application

Acridine Orange/Propidium Iodide Stain for Cell Count and Viability

Protocol Overview

2 µl of the stain is mixed with 18 µl of the cell suspension. Cells with stain are loaded to PhotonSlide and imaged using LUNA-FL.

Tips

Some cells start to die in the chip, good to do the counting as fast as possible.

Features Summary

Easy to focus and export data

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Fast and robust to use, data export

The Bad

Data saving takes a bit long, slides (especially the bright field slides) have some stripes (don't affect the counting)

The Bottom Line

The LUNA-FL Dual Fluorescence Cell Counter is easy to use and produces fast and reliable results. We have found it to be very helpful in performing fast quality control prior single cell RNA-seq analysis.

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