Immunohistochemistry with GFAP

August 30, 2018

BlueskyReddit
Neurology
University of Alabama at Birmingham
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

DAKO / Agilent

Product Name:

Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein

Catalog Number:

Z033429-2

GFAP is an intracytoplasmic filamentous protein and makes up the cytoskeleton in astrocytes. It is proven to be a specific marker for cells of astrocytic origin. In conclusion - very satisfied with the pricing and ease to use.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse Brain Sections - Free floating

Primary Incubation

rb GFAP 1:4000 in 1% donkey serum in TBST (0.25% TX100)

Blocking Agent

5% donkey serum in TBST (0.25% TX100)

Secondary Incubation

donkey anti-rb FITC 1:1000 in 1% donkey serum in TBST (0.25% TX100)

Tertiary Incubation

N/A

Detection

Fluoresence

Results Summary

Cells of astrocytic origin show staining within the cytoplasm

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly reliable and cheap

The Bad

Optimization may be needed for different species

The Bottom Line

This is a gold-standard antibody for any experiment directed to studying the morphology & function of astrocytes. Very satisfied with the pricing and ease to use.

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