Santa Cruz
HA-probe Antibody (F-7)
sc-7392
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: HA Antibodies
This antibody was used for immunofluorescence on HeLa and HEK293 cells transiently transfected with a HA-fused protein, to evaluate the localisation of the HA-fused protein transfected.
Immunofluorescence
Cell line
Antibody diluted 1:75 in blocking solution, incubated for 2 hrs at RT
BSA 2%
Goat anti-mouse AlexaFluor 555 (A28180) diluted 1:200 in blocking solution 1 hr at RT
N/A
Olympus FluoView FV1000
The antibody works well in immunofluorescence on paraformaldehyde fixed cells. Low background as compared to stain performed on non transfected cells.
Specific stain
At higher dilution, the signal is lower
The antibody is good for immunofluorescence. It was also specific in western blot.