Excellent Antibody Against Human Beta 1 Integrin

August 06, 2018

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

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Company:

Millipore

Product Name:

Anti-Integrin beta1 Antibody

Catalog Number:

MAB1965

Integrins are cell surface transmembrane molecules that primarily mediate adhesion of cells to matrix proteins and induce cell signaling. Among integrins, beta 1 integrins play crucial roles in migration of normal and cancer cells. Beta 1 integrins are also involved in invasion and metastasis of cancer cells. We analyzed the levels of beta 1 integrin expression in human tumor cells exhibiting different dissemination potentials. To achieve this goal, we compared several anti-integrin beta 1 antibodies, including MAB1965, for their ability to recognize beta 1 integrin in western blotting under reducing and non-reducing conditions.

Experimental Design and Results Summary

Applications

Western Blot

Sample

lysates of human tumor cells; non-reducing conditions-20 microgram per lane; reducing conditions-40 microgram per lane

Primary Incubation

Antibody at a 1:1,000 dilution, ON incubation with gentle rocking in a cold room

Blocking Agent

5% BSA in PBS-0.05% Tween; 45-60 min, RT, gentle rocking

Secondary Incubation

HRP-conjugated anti-mouse antibody at 1:5,000 in 5% milk in PBS-0.05% Tween 20

Tertiary Incubation

N/A

Detection

West Pico

Results Summary

The MAB1965 specifically detected one protein band with an apparent mol. wt. of ~115-130 kDa, which is expected for human beta 1 integrin. The high specificity of this antibody was confirmed by a significantly reduced signal in the cells in which beta 1 integrin was stably knocked down with beta 1-specific shRNA (beta 1-KD) compared with the signal intensity in control cells (Ctrl). The MAB1965 antibody detects beta 1 integrin under both reducing (Red.) and non-reducing conditions (Non-Reduced).

DOI or PMID #

N/A

Additional Notes

Detection of beta 1 integrin under reducing conditions would require higher protein load per lane compared to non-reducing conditions.

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Summary

The Good

The MAB1965 is a rare monoclonal antibody that detects beta 1 integrin under both reducing and non-reducing conditions.

The Bad

None detected.

The Bottom Line

The antibody MAB1965 is a very sensitive mouse monoclonal antibody that specifically recognizes human beta 1 integrin.

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