Agilent Technologies
QuikChange II XL Site-Directed Mutagenesis Kit
200521-5
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Our lab works on TCR engineering of CD8 T cells to target HIV-infected cells for elimination via lentiviral transductions. As part of complicated studies to understand the role of the TCR in HIV control, or how different mutants with different chain pairing can affect activity, we have utilized with kit to generate mutant sequences of the TCRs.
Mutagenesis
DNA sequence encoding TCRS
We strictly follow the kit instructions without modifications
Make sure to start with high quality DNA
Good kit. Can also get larger ones for more reactions (this one is for 10 only)
N/A
Works well
None
I recommend it.