Benchtop Cytometer for Cell Cycle Analysis

November 30, 2018

University of Kansas Medical Center
Pathology
Postdoctoral Fellow

Overall

Performance

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Guava easyCyte 6HT-2L

Catalog Number:

0500-4007

Our research interests include understanding the mechanisms underlying use of kinesin inhibitors as treatments for cancer. In these assays, we sought to determine cell cycle distribution following treatment with an Eg5 inhibitor, ispinesib, in ovarian cancer cell lines.

Experimental Design and Results Summary

Application

Flow Cytometry/Cell Cycle Analysis

Protocol Overview

Cell were treated with ispinesib, allowed to grow for 12-48 hours, then fixed and stained using the Guava Cell Cycle Reagent according to manufacturer's protocol. Data were acquired using the Guava instrument and software.

Tips

Be careful to regularly clean the instrument and to run QC checks often. Often we return to use the istrument after a break in use to find it not working (e.g. clogged). Also, it may be useful to have a person who monitors the instrument to ensure lasers are turned off after use.

Features Summary

Software allows for easy data analysis and generations of charts and graphs

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Excellent for bench-top cell cycle analysis, when it is maintained and barring any glitches

The Bad

Requires quite a bit of oversight to ensure proper operation

The Bottom Line

Nice instrument that is intuitive and easy to use, but may require too much oversight - especially in smaller lab groups without a dedicated lab manager.

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