Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb 8516S from Cell Signaling Technology

Product Specs
ItemPhospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $448.00 Inquire
Catalog Number8516S
Quantity100 µl
ApplicationsW, W-S, IP, IHC-P, IF-F, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetRb (Ser807/Ser811) phosphate
IsotypeIgG
ModificationsRb (Ser807/Ser811) phosphate
HostRabbit
NCBI Full Gene NameRB transcriptional corepressor 1
CloneD20B12
Molecular Weight110
ClonalityMonoclonal
NCBI Gene AliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
Research AreaCell Cycle / Checkpoint Control

Questions and Answers

Can this antibody be used to study the phosphorylation status of Rb in different cancer types?

Yes, the Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb has been utilized in various studies to assess the phosphorylation status of the Rb protein, particularly in cancer contexts. For instance, it has been referenced in research involving hepatocellular carcinoma and malignant peripheral nerve sheath tumors, where Rb phosphorylation plays a critical role in cell cycle regulation and tumor progression (, ).

Is this antibody effective for immunofluorescence applications?

The Phospho-Rb (Ser807/811) antibody has been shown to work effectively in immunofluorescence applications, as demonstrated in studies where it was used to visualize phosphorylated Rb in various cell types, including cardiac endothelial cells and gastric cancer models (, ).

What species can this antibody be used with?

This antibody is reactive with multiple species, including human, mouse, rat, and non-human primates, making it versatile for various experimental models in cell biology and cancer research.

Are there any specific experimental conditions or dilutions recommended for using this antibody in Western blotting?

For Western blotting, the antibody is typically used at a dilution of 1:2000, as indicated in several studies that have successfully employed it to detect phosphorylated Rb in various cell lines and tissue samples (, ).

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) PIAS4 inhibition induces cell cycle arrest and exhibits a synergistic effect in combination with CDK4/6 inhibitor in breast cancer treatmentOncogeneMay 26, 2026Hui Chen, Xinyang Hu, Lifeng Feng, Xiaoqing Cheng, Liyuan Zhu, Fei Xu, Tao Zhu, Yongmei Gao, Jin Wang, Hongchuan Jin, et al.used were sourced as follows: anti-PIAS4 (4392, CST), anti-PIAS4 (ab58416, Abcam), anti-p807/811-RB1 (8516, CST), anti-p780-RB1 (9307, CST), anti-p795-RB1 (9301, CST), anti-RB1 (10048-2-Ig, Proteintech), anti-pY24-CDK6

(2) Intravesical Delivery of P21 mRNA–Loaded Lipid Nanoparticles as a Tumor Suppressor Replacement Therapy for Bladder CancerFASEB JMay 17, 2026Jie Zeng, Ziyi Cao, Chenghe Wang, Yingjie XuAnti‐p21 (CST, 2947, 1:1000), anti‐p21 (Abcam, ab188224, 1:1000), anti‐phospho‐Rb (Ser807/811) (CST, 8516, 1:1000), anti‐Cyclin E (CST, 20808, 1:1000), anti‐Cyclin B (CST, 12231, 1:1000), anti‐PARP1 (CST, 9542

(3) Dormancy exit correlates with CREB1 reactivation that is a potential target to overcome endocrine therapy resistance in ER+ breast cancer cellsiScienceMay 15, 2026Lei Duan, Kelsey M. O’Hara, Carl G. MakiAB_1903940 Rabbit Monoclonal Anti-phospho-Rb (Ser807/811) (D20B12),Cell Signaling Technology,Cat##8516 RRID: AB_331472 Mouse Monoclonal Anti-Rb (4H1),Cell Signaling Technology,Cat##9309 RRID: AB_823629 Rabbit

(4) The Cyclin C-CDK8/19 Mediator kinase module controls PRCC-TFE3 driven senescence in renal epithelium and tumorigenesis in TFE3-RCCNeoplasiaMay 1, 2026Shoichiro Kuroda, Shintaro Funasaki, Hidekazu Nishizawa, Laura S. Schmidt, Ryoma Kurahashi, Takaaki Ito, Yuichiro Arima, Miwa Tanaka, Atsuya Kitada, Amy M. James, et al.(4H1; #9309; Cell Signaling Technology), phospho-Rb (Ser780; #8180; CST), phospho-Rb (Ser807/811; #8516; CST), p16 (#92803; CST), p21 (#2947; CST), p53 (sc-99; Santa Cruz Biotechnology), CDK2 (#2546; CST),

(5) Insulinoma-associated 1 promotes neurogenic proliferation of cortical basal progenitors but is largely dispensable for projection neuron productionbioRxivApril 6, 2026Venkata Thulabandu, Xinwei Cao1:100), FOXP2 (Synaptic Systems 485005, 1:200), Phospho-Rb (Ser807/811) (Cell Signaling Technologies #8516, 1:100). EdU staining was performed using the Click-iT Plus EdU kit (Thermo Fisher Scientific, C10638)

(6) CDK12/CDK13 inhibition disrupts transcriptional elongation and replication fork progression in glioblastomaEMBO Molecular MedicineMarch 25, 2026Silje Lier, Sara B Markusson, Anja Kocijancic, Martine Narum, Solveig O Lund, Bianka Böllering, Anuja Lipsa, Mirra L C Søegaard, Idun D Rein, Petra Santha, et al.IgG,Thermo Fisher Scientific,A11029 Rabbit monoclonal anti-phospho-Rb (Ser807/811),Cell signaling,8516 s Rabbit monoclonal anti-phospho-FoxM Thr600,Cell signaling,14655S Rabbit monoclonal anti-phospho-histone H2A.X (Ser139

(7) Protocol for iterative indirect immunofluorescence imaging of frozen mouse intestinal tissuesSTAR ProtocMarch 20, 2026Shuji Matsuguchi, Aiyan Yau, Ayaka Omori, Takaya Abe, Hiroshi Kiyonari, Yumi KonagayaRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Phospho-Rb (Ser807/811) (D20B12) (dilution 1:2500),Cell signaling Technology,Cat#8516S; RRID:AB_11178658 Phospho-Smad1/5/8 (Ser463/465) (dilution 1:50),Merck,Cat#AB3848-I; RRID:AB_177439 Vimentin

(8) B3GNT3 facilitates NFKB2 processing and non-canonical NF-κB activation to drive lung adenocarcinoma progressionCell Oncol (Dordr)March 16, 2026Ying Lin, Yao Zhang, Bo Yu, Jialei Wang, Zheng Wu, Huijie Wang(51064-2-AP), β-actin (66009-1-Ig) and tubulin (10094-1-AP). Antibodies against pRB (s807/811, CST, 8516), Rb (CST, 9309) and p100 (s866/870, CST, 4810), p100 (CST, 4882) and H3 (CST, 9715) were purchased

(9) An mRNA-encoded scFv antibody targeting the helix-α3 of HPV18 E7 oncoprotein as a novel antiviral strategymBioMarch 11, 2026Feng Han, Xin-ying Guo, Ling-yan Cui, Meng-xuan Zhang, Ya-rong Zeng, Gui-qiang Wang, Jin-jin Li, Xin Chi, Ming-xia Jiang, Yue-ting Xiong, et al.anti-β-actin-HRP (ProteinTech, HRP-60008, 1:5,000), anti-Rb (CST, no.9309S, 1:100), anti- phospho-Rb (CST, no.8516S, 1:100). Protein quantification was analyzed using ImageJ. Immunofluorescence Cells were seeded in

(10) Single-nucleus transcriptomics, pharmacokinetics, and pharmacodynamics of CDK4/6 and mTOR inhibition in a Phase 0/1 trial of recurrent high-grade gliomaNeuro-OncologyMarch 1, 2026Kevin C Johnson, An-Chi Tien, Jun Jiang, James McNamara, Yu-Wei Chang, Chelsea Montgomery, Anita DeSantis, Leonel Elena-Sanchez, Yoko Fujita, Seongho Kim, et al.using immunohistochemistry (IHC) with the following antibodies: anti-pRb (Cell Signaling Technology; #8516, 1:400), anti-pFOXM1 (Cell Signaling Technology; #14655, 1:200), anti-Ki-67 (DAKO; M724029, 1:100);

(11) Ezh2 Delays Activation of Differentiation Genes During Normal Cerebellar Granule Neuron Development and in MedulloblastomabioRxivFebruary 27, 2026James Purzner, Alexander S. Brown, Teresa Purzner, Lauren Ellis, Sara Broski, Ulrike Litzenburger, Kaytlin Andrews, Aryaman Sharma, Xin Wang, Michael D. Taylor, et al.monoclonal,IgG,MAB377,1:200,AB_2298772 Phospho-Rb (Ser807/811) clone D20B12,Cell Signaling Technology,rabbit monoclonal,IgG,8516,1:1000,AB_11178658 p27,Abcam,Rabbit monoclonal,IgG,ab32034,1:100,AB_2244732 Cbx7,Abcam,Rabbit polyclonal,IgG,ab21873,1:200,AB_726005 H3K27me3,Active Motif

(12) Systematic multivariate analysis of chromatin complex dependencies reveals Set1C/COMPASS as a melanoma-enriched epigenetic vulnerabilitybioRxivFebruary 18, 2026Luisa Quesada Camacho, Mohammad Fallahi-SichaniIF, 1:1600), p-RbS807/811 (D20B12) (AB_11178658; rabbit mAb, Cell Signaling Technology, catalog no. 8516, IF, 1:1000), p-RbT373 (rabbit mAb, Abcam, catalog no. ab52975, IF, 1:1000). The following secondary

(13) Hepatocyte-Specific MET Deletion Exacerbates Acetaminophen-Induced Hepatotoxicity in MiceThe American Journal of PathologyFebruary 1, 2026Siddhi Jain, Ranjan Mukherjee, Gillian Williams, Jia-Jun Liu, Lanuza A.P. Faccioli, Zhiping Hu, Rodrigo M. Florentino, George K. Michalopoulos, Alejandro Soto-Gutierrez, Silvia Liu, et al.GCLm (#33381), AIF (#4642), phospho-ERK1/2 (T202/Y204; #9101), ERK1/2 (#4696), phospho-RB (S807/811; #8516), Cyclin-D1 (#55506), PCNA (#2586), IRE1α (#3294), BiP (#3177), VDAC (#4661), GAPDH (#5174), and β-actin

(14) Novel Cell Cycle Inhibitors Decrease Primary and Metastatic Breast Cancer Growth In VivoCancers (Basel)January 30, 2026Mir Shahid Maqbool, Yongzhan Zhang, Karin Strittmatter, Ana Gvozdenovic, Simran Asawa, Masroor A. Paddar, Mukesh Kumar, Umed Singh, Parvinder Pal Singh, Nicola Aceto, et al.with target primary antibodies (p-Rpb1 CTD (Ser2/5) (4735 CS), Rpb1 CTD (2629 CST), p-Rb (Ser807/811) (8516 CST), Rb (4H1) (mouse mAb 9309 CST), and β-actin A3854 (Sigma, Merck KGaA, St. Louis, MO, USA)) and

(15) Transient proliferation by reversible YAP and mitogen control of the cyclin D1/p27 ratioCommunications BiologyJanuary 29, 2026Katherine R. Ferrick, Samsara W. Upadhya, Yilin Fan, Nalin Ratnayeke, Mary N. Teruel, Tobias MeyerCat#2947, RRID:AB_823586), rabbit anti-phospho-Rb(Ser807/811)(1:2500, Cell Signaling Technology Cat#8516, RRID:AB_1117658), rabbit anti-phospho-S6(240/244)(1:2500, Cell Signaling Technology Cat#5364, RRID:AB_10694233),

(16) EZH2 directs HER2+ breast cancer progression through the modulation of epithelial plasticityEMBO ReportsJanuary 28, 2026Linshan Liu, Ellie J Massey, Dongmei Zuo, Alain Pacis, Mert Demirdizen, Elizabeth Podleszanski, Jessica Cinkornpumin, Yu Gu, Hailey Proud, Virginie Sanguin-Gendreau, et al.,Cell Signaling,2978 Rabbit anti-LaminB1,Cell Signaling,17416 Rabbit anti-p-Rb (S807/S811),Cell Signaling,8516 Rabbit anti-CCP3,Cell Signaling,9661 Rabbit anti-p-S6 (S240/244),Cell Signaling,5364 Rabbit anti-p-4EBP1

(17) Paeonia lactiflora Callus-Derived Polynucleotides Enhance Collagen Accumulation in Human Dermal FibroblastsJournal of Functional BiomaterialsJanuary 22, 2026Soyoung Hwang, Seunghye Park, Jin Woo Lee, Mira Park, Le Anh Nguyet, Yongsung Hwang, Keunsun Ahn, Hyun-young Shin, Kuk Hui SonCell Signaling Technology), RB (SC-74570, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), phospho-RB (Ser807/811; #8516S, 1:1000, Cell Signaling Technology), NF-κB (#8242S, 1:1000, Cell Signaling Technology), Histone H3 (#4499S,

(18) Identification and inhibition of the Cyclin D Rb-docking interface that drives cell divisionbioRxivJanuary 21, 2026Benjamin R. Topacio, Cecelia Brown Fleming, Michael C. Lanz, Shuyuan Zhang, Shicong Xie, Jurgen Tuvikene, Aiden Weaver, Ioannis Sanidas, Julien Sage, Seth M. Rubin, et al.the following antibodies: Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Cell Signaling Technology #8516), Phospho-Rb (Ser780) (D59B7) Rabbit mAb (Cell Signaling Technology #8180), Rb (4H1) Mouse mAb (Cell Signaling Technology

(19) Paired primary-metastasis patient-derived organoids and mouse models identify phenotypic evolution and druggable dependencies of peritoneal metastasis from appendiceal cancerbioRxivJanuary 16, 2026Ahmed Mahmoud, Philip H. Choi, Christine Sukhwa, Jura Pintar, Henry Walch, Nan Zhao, Jonathan Bermeo, Sebastian Chung, Manisha Raghavan, Samhita Bapat, et al.RRID:AB_2070801 Rabbit monoclonal anti-phospho-Rb (Ser807/811) (clone D20B12),Cell Signaling Technology,Cat#8516 RRID:AB_11178658 Rabbit polyclonal anti-Cleaved Caspase-3 (Asp175),Cell Signaling Technology,Cat#9661

(20) Therapeutic benefits of maintaining CDK4/6 inhibitors and incorporating CDK2 inhibitors beyond progression in breast cancereLifeDecember 29, 2025Jessica Armand, Sungsoo Kim, Kibum Kim, Eugene Son, Minah Kim, Kevin Kalinsky, Hee Won YangDNA synthesis. Antibodies Primary antibodies were from Cell Signaling Technology: Rb (9309), phospho-Rb (Ser807/811; 8516), and c-Myc (5605); from Abcam: CDK6 (ab124821), cyclin E (ab32103), and cyclin A (ab32386); and from

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7/6/2018Good Phospho-RB Antibody for Western Blot Rebecca WatesWe are interested in mechanisms of resistance to targeted therapies in ovarian cancer. This experiment was designed to determine differential Rb1 protein expression and post-translational ... Read Review

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb from Cell Signaling Technology

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Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb from Cell Signaling Technology

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