Thermal Cycler For PCR

The J. David Gladstone Institutes
Virology and Immunology
Research Associate

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Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

S1000™ Thermal Cycler with Dual 48/48 Fast Reaction Module

Catalog Number:

1852148

Thermal cycler is used for polymerase chain reaction and cDNA synthesis. The machine is dependable and makes reproducible strong bands. This model comes with a variety of functions but the best one is being reliable.

Experimental Design and Results Summary

Application

PCR

Protocol Overview

Thermal cycler is used for PCR with mouse DNA. Mouse tails are digested overnight and then raw DNA is amplified using taq polymerase and other reagents inside the 48 well block. Amplified DNA is then run on a gel.The thermal cycler is also used for cDNA synthesis using a Maxima cDNA synthesis kit.

Tips

Do not leave the thermal cycler overnight at 4 degrees. This causes condensation to fill the blocks. Make sure the thermal cycler has enough airflow around.

Features Summary

There are two 48 well blocks allowing for two programs to run at once. Blocks are detachable. Thermal cycler has two temperature options: temperature and gradient temperature. Maximum time is 18 hours a step and able to do 99 steps with a possible 9999 repeats. There is a simple small screen with buttons for navigation

DOI or PMID #

N/A

Additional Notes

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Summary

The Good

Machine is dependable and a good size to fit on a crowded lab bench.

The Bad

N/A

The Bottom Line

Great model. Neighboring labs are using this product as well.

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