Mouse Endothelial Cell FACS

June 13, 2018

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD31 (PECAM-1) Antibody, PE-Cyanine7 (Monoclonal, 390)

Catalog Number:

25-0311-81

The aim is to stain mouse endothelial cells using this antibody, then analyze it using FACS. The staining was great.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse skin endothelial cells

Primary Incubation

4C 10min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Nearly all cells stained positive as expected. Great staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy and simple staining

The Bad

None

The Bottom Line

It works great, no complaints.

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