Smad3 Antibody

June 01, 2018

BlueskyReddit
Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Smad3 antibody [EP568Y] (Phycoerythrin)

Catalog Number:

ab208751

I used this antibody in a flow cytometry assay to quantify total Smad3 in NK cells and CD8+ T cells. Antibody worked well and gave a bright signal. Was able to titrate it down to use considerably less than what was recommended and still got good results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

1:2000 dilution, 30 min at 4C, cells fixed with BD Fix-Lyse, permeabilized with BD Perm buffer III

Blocking Agent

Fc block (Anti CD16/CD32)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

Good strong signal with very little antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works well in fix/perm mouse blood.

The Bad

Very bright signal, need to titrate down to get optimal results.

The Bottom Line

Very good antibody.

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